Linked Life Data

15056802

Source:http://linkedlifedata.com/resource/pubmed/id/15056802

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:15056802rdf:typepubmed:Citationlld:pubmed
pubmed-article:15056802lifeskim:mentionsumls-concept:C0086418lld:lifeskim
pubmed-article:15056802lifeskim:mentionsumls-concept:C0010453lld:lifeskim
pubmed-article:15056802lifeskim:mentionsumls-concept:C0227525lld:lifeskim
pubmed-article:15056802lifeskim:mentionsumls-concept:C0010763lld:lifeskim
pubmed-article:15056802lifeskim:mentionsumls-concept:C0001962lld:lifeskim
pubmed-article:15056802lifeskim:mentionsumls-concept:C0009002lld:lifeskim
pubmed-article:15056802lifeskim:mentionsumls-concept:C0030234lld:lifeskim
pubmed-article:15056802lifeskim:mentionsumls-concept:C0919426lld:lifeskim
pubmed-article:15056802lifeskim:mentionsumls-concept:C1413886lld:lifeskim
pubmed-article:15056802lifeskim:mentionsumls-concept:C0851285lld:lifeskim
pubmed-article:15056802lifeskim:mentionsumls-concept:C0205225lld:lifeskim
pubmed-article:15056802pubmed:issue2lld:pubmed
pubmed-article:15056802pubmed:dateCreated2004-5-20lld:pubmed
pubmed-article:15056802pubmed:abstractTextCYP2E1 and CYP4A11 are cytochrome P450 enzymes that are regulated by physiological conditions including diabetes and fasting. In addition, the xenochemical clofibrate has been reported to induce both rodent CYP2E1 and CYP4A. These findings suggest similar modes of regulation. Also in common to both enzymes is the ability to metabolize fatty acids such as laurate and arachidonic acid. Here, we used primary cultures of human hepatocytes to determine if certain xenochemicals could regulate CYP2E1 and CYP4A11. Ethanol significantly (p < 0.05) increased expression of CYP2E1 mRNA by 216 +/- 32% of control, but did not alter CYP4A11 mRNA accumulation (145 +/- 22% of control). In contrast, hepatocytes exposed to ethanol exhibited only a slight elevation in CYP2E1 protein (122 +/- 13% of control) and a negligible effect on CYP4A11 protein. Clofibrate significantly (p < 0.05) enhanced both CYP4A11 mRNA and protein by 239 +/- 30% and 154 +/- 10% of control, respectively, but did not increase CYP2E1. Because rodent CYP4A is reportedly regulated by fatty acids through peroxisome proliferator activated receptor alpha (PPARalpha) and CYP2E1 is induced by high fat diets, we examined the effects of a medium chain fatty acid, palmitate on CYP2E1 mRNA content. Palmitic acid significantly (p < 0.05) increased CYP2E1 mRNA to 326 +/- 57% of control. Collectively, results presented here identify agents that enhance CYP2E1 and CYP4A11 at the transcription level and suggest that fatty acids may represent a similar mode of regulation for these P450 enzymes. The lack of induction of CYP2E1 protein by ethanol in human hepatocytes indicates that for certain P450 enzymes, isolated hepatocytes may not be an adequate tool for predicting in vivo responses.lld:pubmed
pubmed-article:15056802pubmed:granthttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:15056802pubmed:granthttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:15056802pubmed:granthttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:15056802pubmed:languageenglld:pubmed
pubmed-article:15056802pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:15056802pubmed:citationSubsetIMlld:pubmed
pubmed-article:15056802pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:15056802pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:15056802pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:15056802pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:15056802pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:15056802pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:15056802pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:15056802pubmed:statusMEDLINElld:pubmed
pubmed-article:15056802pubmed:monthJunlld:pubmed
pubmed-article:15056802pubmed:issn1096-6080lld:pubmed
pubmed-article:15056802pubmed:authorpubmed-author:RaucyJudy LJLlld:pubmed
pubmed-article:15056802pubmed:authorpubmed-author:LaskerJeromeJlld:pubmed
pubmed-article:15056802pubmed:authorpubmed-author:OzakiKazuakiKlld:pubmed
pubmed-article:15056802pubmed:authorpubmed-author:ZoletaVeronic...lld:pubmed
pubmed-article:15056802pubmed:issnTypePrintlld:pubmed
pubmed-article:15056802pubmed:volume79lld:pubmed
pubmed-article:15056802pubmed:ownerNLMlld:pubmed
pubmed-article:15056802pubmed:authorsCompleteYlld:pubmed
pubmed-article:15056802pubmed:pagination233-41lld:pubmed
pubmed-article:15056802pubmed:dateRevised2010-9-17lld:pubmed
pubmed-article:15056802pubmed:meshHeadingpubmed-meshheading:15056802...lld:pubmed
pubmed-article:15056802pubmed:meshHeadingpubmed-meshheading:15056802...lld:pubmed
pubmed-article:15056802pubmed:meshHeadingpubmed-meshheading:15056802...lld:pubmed
pubmed-article:15056802pubmed:meshHeadingpubmed-meshheading:15056802...lld:pubmed
pubmed-article:15056802pubmed:meshHeadingpubmed-meshheading:15056802...lld:pubmed
pubmed-article:15056802pubmed:meshHeadingpubmed-meshheading:15056802...lld:pubmed
pubmed-article:15056802pubmed:meshHeadingpubmed-meshheading:15056802...lld:pubmed
pubmed-article:15056802pubmed:meshHeadingpubmed-meshheading:15056802...lld:pubmed
pubmed-article:15056802pubmed:meshHeadingpubmed-meshheading:15056802...lld:pubmed
pubmed-article:15056802pubmed:meshHeadingpubmed-meshheading:15056802...lld:pubmed
pubmed-article:15056802pubmed:meshHeadingpubmed-meshheading:15056802...lld:pubmed
pubmed-article:15056802pubmed:meshHeadingpubmed-meshheading:15056802...lld:pubmed
pubmed-article:15056802pubmed:meshHeadingpubmed-meshheading:15056802...lld:pubmed
pubmed-article:15056802pubmed:meshHeadingpubmed-meshheading:15056802...lld:pubmed
pubmed-article:15056802pubmed:meshHeadingpubmed-meshheading:15056802...lld:pubmed
pubmed-article:15056802pubmed:meshHeadingpubmed-meshheading:15056802...lld:pubmed
pubmed-article:15056802pubmed:meshHeadingpubmed-meshheading:15056802...lld:pubmed
pubmed-article:15056802pubmed:meshHeadingpubmed-meshheading:15056802...lld:pubmed
pubmed-article:15056802pubmed:meshHeadingpubmed-meshheading:15056802...lld:pubmed
pubmed-article:15056802pubmed:meshHeadingpubmed-meshheading:15056802...lld:pubmed
pubmed-article:15056802pubmed:year2004lld:pubmed
pubmed-article:15056802pubmed:articleTitleRegulation of CYP2E1 by ethanol and palmitic acid and CYP4A11 by clofibrate in primary cultures of human hepatocytes.lld:pubmed
pubmed-article:15056802pubmed:affiliationCalifornia Toxicology Research Institute, 1989 Palomar Oaks Way, Suite B, Carlsbad, California 92009, USA. jraucy@ctri-np.orglld:pubmed
pubmed-article:15056802pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:15056802pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:15056802lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:15056802lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:15056802lld:pubmed