| pubmed-article:15044082 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15044082 | lifeskim:mentions | umls-concept:C0002092 | lld:lifeskim |
| pubmed-article:15044082 | lifeskim:mentions | umls-concept:C0085080 | lld:lifeskim |
| pubmed-article:15044082 | lifeskim:mentions | umls-concept:C0009221 | lld:lifeskim |
| pubmed-article:15044082 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:15044082 | lifeskim:mentions | umls-concept:C0998367 | lld:lifeskim |
| pubmed-article:15044082 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:15044082 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:15044082 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:15044082 | pubmed:dateCreated | 2004-3-26 | lld:pubmed |
| pubmed-article:15044082 | pubmed:abstractText | Blo t 5 is a major allergen from house dust mite Blomia tropicalis. Purification of native Blo t 5 (nBlo t 5) from whole dust mite extract is tedious and gave low yield. In this study, we demonstrated that codon optimization facilitated high-level expression of Blo t 5 in Chinese hamster ovary (CHO)-K1 cells and thus allows production of sufficient recombinant cBlo t 5 for specific immunotherapy. A codon optimized Blo t 5 gene was synthesized by PCR and the codon optimized or wild-type Blo t 5 gene in pcDNA3.0 was transfected into CHO-K1 cells and stably selected with Geneticin (G418). Western-immunoblot analysis of spent culture media detected a positive band at 14kDa for the codon optimized but not wild-type gene transfectants. In addition, a stable CHO-K1 clone produced up to 13 mg/L of the cBlo t 5 protein having a high correlation of human IgE reactivities and allergenicity to the native Blo t 5, thus indicating proper conformation of this recombinant form. | lld:pubmed |
| pubmed-article:15044082 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15044082 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15044082 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15044082 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15044082 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15044082 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15044082 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15044082 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15044082 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:15044082 | pubmed:issn | 0006-291X | lld:pubmed |
| pubmed-article:15044082 | pubmed:author | pubmed-author:RoyW AWA | lld:pubmed |
| pubmed-article:15044082 | pubmed:author | pubmed-author:MerC LCL | lld:pubmed |
| pubmed-article:15044082 | pubmed:author | pubmed-author:HOCKC WCW | lld:pubmed |
| pubmed-article:15044082 | pubmed:author | pubmed-author:CheongNN | lld:pubmed |
| pubmed-article:15044082 | pubmed:author | pubmed-author:LiH YHY | lld:pubmed |
| pubmed-article:15044082 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15044082 | pubmed:day | 16 | lld:pubmed |
| pubmed-article:15044082 | pubmed:volume | 316 | lld:pubmed |
| pubmed-article:15044082 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15044082 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15044082 | pubmed:pagination | 991-6 | lld:pubmed |
| pubmed-article:15044082 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:15044082 | pubmed:meshHeading | pubmed-meshheading:15044082... | lld:pubmed |
| pubmed-article:15044082 | pubmed:meshHeading | pubmed-meshheading:15044082... | lld:pubmed |
| pubmed-article:15044082 | pubmed:meshHeading | pubmed-meshheading:15044082... | lld:pubmed |
| pubmed-article:15044082 | pubmed:meshHeading | pubmed-meshheading:15044082... | lld:pubmed |
| pubmed-article:15044082 | pubmed:meshHeading | pubmed-meshheading:15044082... | lld:pubmed |
| pubmed-article:15044082 | pubmed:meshHeading | pubmed-meshheading:15044082... | lld:pubmed |
| pubmed-article:15044082 | pubmed:meshHeading | pubmed-meshheading:15044082... | lld:pubmed |
| pubmed-article:15044082 | pubmed:meshHeading | pubmed-meshheading:15044082... | lld:pubmed |
| pubmed-article:15044082 | pubmed:meshHeading | pubmed-meshheading:15044082... | lld:pubmed |
| pubmed-article:15044082 | pubmed:meshHeading | pubmed-meshheading:15044082... | lld:pubmed |
| pubmed-article:15044082 | pubmed:meshHeading | pubmed-meshheading:15044082... | lld:pubmed |
| pubmed-article:15044082 | pubmed:meshHeading | pubmed-meshheading:15044082... | lld:pubmed |
| pubmed-article:15044082 | pubmed:meshHeading | pubmed-meshheading:15044082... | lld:pubmed |
| pubmed-article:15044082 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15044082 | pubmed:articleTitle | High-level expression of a codon optimized recombinant dust mite allergen, Blo t 5, in Chinese hamster ovary cells. | lld:pubmed |
| pubmed-article:15044082 | pubmed:affiliation | Department of Paediatrics, National University of Singapore, Singapore. | lld:pubmed |
| pubmed-article:15044082 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15044082 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15044082 | lld:pubmed |
