| pubmed-article:1503776 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1503776 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:1503776 | lifeskim:mentions | umls-concept:C0439659 | lld:lifeskim |
| pubmed-article:1503776 | lifeskim:mentions | umls-concept:C1705822 | lld:lifeskim |
| pubmed-article:1503776 | lifeskim:mentions | umls-concept:C0332256 | lld:lifeskim |
| pubmed-article:1503776 | lifeskim:mentions | umls-concept:C0348011 | lld:lifeskim |
| pubmed-article:1503776 | lifeskim:mentions | umls-concept:C2347567 | lld:lifeskim |
| pubmed-article:1503776 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:1503776 | pubmed:dateCreated | 1992-9-21 | lld:pubmed |
| pubmed-article:1503776 | pubmed:abstractText | Plasmid constructs are described that carry retrievable DNA cassettes containing the origin of transfer region (oriT) from two broad-host-range plasmids. Restriction of these high copy number plasmids with any one of a variety of enzymes yields a linear DNA fragment of convenient size containing the oriT region of either pCUI or RK2. This DNA can be ligated into any vector or recombinant plasmid containing a compatible enzyme site and can be easily identified by size on an agarose gel. Any plasmid can therefore be mobilized using a number of helper strains or conjugative plasmids derived from the parental plasmids. In addition, the cassettes can be used for a variety of genetic manipulations including "selectable" linker mutagenesis. | lld:pubmed |
| pubmed-article:1503776 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1503776 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1503776 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1503776 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1503776 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1503776 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:1503776 | pubmed:issn | 0736-6205 | lld:pubmed |
| pubmed-article:1503776 | pubmed:author | pubmed-author:IyerV NVN | lld:pubmed |
| pubmed-article:1503776 | pubmed:author | pubmed-author:HengenP NPN | lld:pubmed |
| pubmed-article:1503776 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1503776 | pubmed:volume | 13 | lld:pubmed |
| pubmed-article:1503776 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1503776 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1503776 | pubmed:pagination | 56-8, 60, 62 | lld:pubmed |
| pubmed-article:1503776 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:1503776 | pubmed:meshHeading | pubmed-meshheading:1503776-... | lld:pubmed |
| pubmed-article:1503776 | pubmed:meshHeading | pubmed-meshheading:1503776-... | lld:pubmed |
| pubmed-article:1503776 | pubmed:meshHeading | pubmed-meshheading:1503776-... | lld:pubmed |
| pubmed-article:1503776 | pubmed:meshHeading | pubmed-meshheading:1503776-... | lld:pubmed |
| pubmed-article:1503776 | pubmed:meshHeading | pubmed-meshheading:1503776-... | lld:pubmed |
| pubmed-article:1503776 | pubmed:meshHeading | pubmed-meshheading:1503776-... | lld:pubmed |
| pubmed-article:1503776 | pubmed:meshHeading | pubmed-meshheading:1503776-... | lld:pubmed |
| pubmed-article:1503776 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1503776 | pubmed:articleTitle | DNA cassettes containing the origin of transfer (oriT) of two broad-host-range transfer systems. | lld:pubmed |
| pubmed-article:1503776 | pubmed:affiliation | Department of Biology, Carleton University, Ottawa, Ontario, Canada. | lld:pubmed |
| pubmed-article:1503776 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1503776 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1503776 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1503776 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1503776 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1503776 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1503776 | lld:pubmed |
