| pubmed-article:15036525 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15036525 | lifeskim:mentions | umls-concept:C1260940 | lld:lifeskim |
| pubmed-article:15036525 | lifeskim:mentions | umls-concept:C2756969 | lld:lifeskim |
| pubmed-article:15036525 | lifeskim:mentions | umls-concept:C0016701 | lld:lifeskim |
| pubmed-article:15036525 | lifeskim:mentions | umls-concept:C1516084 | lld:lifeskim |
| pubmed-article:15036525 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:15036525 | pubmed:dateCreated | 2004-3-23 | lld:pubmed |
| pubmed-article:15036525 | pubmed:abstractText | Semen of Greylag (Anser anser L.) ganders was frozen according to a method previously elaborated by the authors for freezing the White Koluda gander semen. Semen was collected from five to eight Greylag ganders, twice a week during three succeeding reproductive cycles, by dorso-abdominal massage. Semen samples were diluted in the ratio of 1:1 or 2:1 (two parts semen: one part diluent) with EK diluent, supplemented by 6% DMF, equilibrated and pre-frozen to -140 degrees C at a rate 60 degrees C/min, before being transferred into liquid nitrogen container. Semen samples thawed in a water bath of 60 degrees C were used for twice a week insemination in a volume of 200 microl. Three Greylag and three White Koluda geese were involved in frozen-thawed semen fertilizing ability test. The reproductive cycle of wild geese lasts usually about 6-7 weeks. The ejaculate volume (30-140 microl) and sperm concentration (10x10(6) to 150x10(6) ml(-1)) are much lower than these of domestic ganders, but spermatozoa morphology is similar, particularly while compared to 1-year-old White Koluda ganders semen. There are about 90% of live spermatozoa and about 30% of live morphologically normal cells in Greylag gander fresh semen. The Greylag gander spermatozoa susceptibility to cryopreservation procedure is as high as in domestic ganders. Dilution ratio 2:1 resulted in higher number of live spermatozoa, which withstood cryoinjury stress. In relation to fresh semen about 60% of spermatozoa remained intact (on the basis of light microscope examination) in the frozen-thawed semen. Insemination of frozen-thawed semen resulted in 37.5% of fertile eggs in Greylag and 25.0% in White Koluda geese. Low fertility rate was caused by an insufficient number of live normal spermatozoa used for insemination (about three million in every dose). | lld:pubmed |
| pubmed-article:15036525 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15036525 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15036525 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15036525 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15036525 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:15036525 | pubmed:issn | 0378-4320 | lld:pubmed |
| pubmed-article:15036525 | pubmed:author | pubmed-author:ChrzanowskaMM | lld:pubmed |
| pubmed-article:15036525 | pubmed:author | pubmed-author:?ukaszewiczEE | lld:pubmed |
| pubmed-article:15036525 | pubmed:author | pubmed-author:JeryszAA | lld:pubmed |
| pubmed-article:15036525 | pubmed:author | pubmed-author:Che?mo?skaBB | lld:pubmed |
| pubmed-article:15036525 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15036525 | pubmed:volume | 80 | lld:pubmed |
| pubmed-article:15036525 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15036525 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15036525 | pubmed:pagination | 163-73 | lld:pubmed |
| pubmed-article:15036525 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:15036525 | pubmed:meshHeading | pubmed-meshheading:15036525... | lld:pubmed |
| pubmed-article:15036525 | pubmed:meshHeading | pubmed-meshheading:15036525... | lld:pubmed |
| pubmed-article:15036525 | pubmed:meshHeading | pubmed-meshheading:15036525... | lld:pubmed |
| pubmed-article:15036525 | pubmed:meshHeading | pubmed-meshheading:15036525... | lld:pubmed |
| pubmed-article:15036525 | pubmed:meshHeading | pubmed-meshheading:15036525... | lld:pubmed |
| pubmed-article:15036525 | pubmed:meshHeading | pubmed-meshheading:15036525... | lld:pubmed |
| pubmed-article:15036525 | pubmed:meshHeading | pubmed-meshheading:15036525... | lld:pubmed |
| pubmed-article:15036525 | pubmed:meshHeading | pubmed-meshheading:15036525... | lld:pubmed |
| pubmed-article:15036525 | pubmed:meshHeading | pubmed-meshheading:15036525... | lld:pubmed |
| pubmed-article:15036525 | pubmed:meshHeading | pubmed-meshheading:15036525... | lld:pubmed |
| pubmed-article:15036525 | pubmed:meshHeading | pubmed-meshheading:15036525... | lld:pubmed |
| pubmed-article:15036525 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15036525 | pubmed:articleTitle | Attempts on freezing the Greylag (Anser anser L.) gander semen. | lld:pubmed |
| pubmed-article:15036525 | pubmed:affiliation | Department of Poultry Breeding, Institute of Animal Breeding, Agricultural University of Wroc?aw, 51-630 Wroc?aw, Poland. ewa@gen.ar.wroc.pl | lld:pubmed |
| pubmed-article:15036525 | pubmed:publicationType | Journal Article | lld:pubmed |
