| pubmed-article:15030527 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15030527 | lifeskim:mentions | umls-concept:C0007427 | lld:lifeskim |
| pubmed-article:15030527 | lifeskim:mentions | umls-concept:C0021390 | lld:lifeskim |
| pubmed-article:15030527 | lifeskim:mentions | umls-concept:C0024432 | lld:lifeskim |
| pubmed-article:15030527 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:15030527 | pubmed:dateCreated | 2004-3-19 | lld:pubmed |
| pubmed-article:15030527 | pubmed:abstractText | Down-regulation of receptors involved in the recognition or transmission of inflammatory signals and a reduced responsiveness support the concept that macrophages are 'desensitized' during their differentiation in the intestinal mucosa. During inflammatory bowel disease (IBD) intestinal macrophages (IMACs) change to a reactive or 'aggressive' type. After having established a method of isolation and purification of IMACs, message for cathepsin D was one of the mRNAs we found to be up-regulated in a subtractive hybridization of Crohn's disease (CD) macrophages versus IMACs from control mucosa. The expression of cathepsin D in intestinal mucosa was analysed by immunohistochemistry in biopsies from IBD and control patients and in a mouse model of dextran sulphate sodium (DSS)-induced acute and chronic colitis. IMACs were isolated and purified from normal and inflamed mucosa by immunomagnetic beads armed with a CD33 antibody. RT-PCR was performed for cathepsin D mRNA. Results were confirmed by Northern blot and flow cytometrical analysis. Immunohistochemistry revealed a significant increase in the cathepsin D protein expression in inflamed intestinal mucosa from IBD patients compared to non-inflamed mucosa. No cathepsin D polymerase chain reaction (PCR) product could be obtained with mRNA from CD33-positive IMACs from normal mucosa. Reverse transcription (RT)-PCR showed an induction of mRNA for cathepsin D in purified IMACs from IBD patients. Northern blot and flow cytometry analysis confirmed these results. Cathepsin D protein was also found in intestinal mucosa in acute and chronic DSS-colitis but was absent in normal mucosa. This study shows that expression of cathepsin D is induced in inflammation-associated IMACs. The presence of cathepsin D might contribute to the mucosal damage in IBD. | lld:pubmed |
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| pubmed-article:15030527 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15030527 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15030527 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15030527 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:15030527 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15030527 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:15030527 | pubmed:issn | 0009-9104 | lld:pubmed |
| pubmed-article:15030527 | pubmed:author | pubmed-author:FalkWW | lld:pubmed |
| pubmed-article:15030527 | pubmed:author | pubmed-author:SchreiterKK | lld:pubmed |
| pubmed-article:15030527 | pubmed:author | pubmed-author:SchölmerichJJ | lld:pubmed |
| pubmed-article:15030527 | pubmed:author | pubmed-author:ObermeierFF | lld:pubmed |
| pubmed-article:15030527 | pubmed:author | pubmed-author:HausmannMM | lld:pubmed |
| pubmed-article:15030527 | pubmed:author | pubmed-author:RoglerGG | lld:pubmed |
| pubmed-article:15030527 | pubmed:author | pubmed-author:HerfarthHH | lld:pubmed |
| pubmed-article:15030527 | pubmed:author | pubmed-author:SaftigPP | lld:pubmed |
| pubmed-article:15030527 | pubmed:author | pubmed-author:SpottlTT | lld:pubmed |
| pubmed-article:15030527 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15030527 | pubmed:volume | 136 | lld:pubmed |
| pubmed-article:15030527 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15030527 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15030527 | pubmed:pagination | 157-67 | lld:pubmed |
| pubmed-article:15030527 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:15030527 | pubmed:meshHeading | pubmed-meshheading:15030527... | lld:pubmed |
| pubmed-article:15030527 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15030527 | pubmed:articleTitle | Cathepsin D is up-regulated in inflammatory bowel disease macrophages. | lld:pubmed |
| pubmed-article:15030527 | pubmed:affiliation | Department of Internal Medicine I, University of Regensburg, Regensburg, Germany. martin.hausmann@klinik.uni-regensburg.de | lld:pubmed |
| pubmed-article:15030527 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15030527 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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