| pubmed-article:15016615 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15016615 | lifeskim:mentions | umls-concept:C0011928 | lld:lifeskim |
| pubmed-article:15016615 | lifeskim:mentions | umls-concept:C0027882 | lld:lifeskim |
| pubmed-article:15016615 | lifeskim:mentions | umls-concept:C0026724 | lld:lifeskim |
| pubmed-article:15016615 | lifeskim:mentions | umls-concept:C1622891 | lld:lifeskim |
| pubmed-article:15016615 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:15016615 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:15016615 | lifeskim:mentions | umls-concept:C0591833 | lld:lifeskim |
| pubmed-article:15016615 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:15016615 | pubmed:dateCreated | 2004-6-14 | lld:pubmed |
| pubmed-article:15016615 | pubmed:abstractText | Upregulation of CGRP-immunoreactive (IR) primary afferent nerve fibers accompanied by mastocytosis is characteristic for the Schistosoma mansoni-infected murine ileum. These mucosal mast cells (MMC) and CGRP-IR fibers, which originate from dorsal root (DRG) and nodose ganglia, are found in close apposition. We examined interactions between primary cultured MMC and CGRP-IR DRG neurons in vitro by confocal recording of intracellular Ca(2+) concentration ([Ca(2+)](i)). The degranulatory EC(50) for the mast cell secretagogue compound 48/80 (C48/80; 10 microg/ml) and the neuropeptides CGRP (2.10(-8) M) and substance P (SP; 3.10(-8) M) were determined by measurement of extracellular release of the granule chymase, mouse mast cell protease-1. Application of C48/80 (10 microg/ml) and CGRP and SP (both 10(-7) M) to Fluo-4-loaded MMC induced a transient rise in [Ca(2+)](i) after a lag time, indicative of mast cell degranulation and/or secretion. The CGRP response could be completely blocked by pertussis toxin (2 microg/ml), indicating involvement of G(i) proteins. Application of MMC juice, obtained by C48/80 degranulation of MMC, to Fluo-4-loaded DRG neurons induced in all neurons a rise in [Ca(2+)](i), indicative of activation. Degranulation of MMC by C48/80 in culture dishes containing Fluo-4-loaded DRG neurons also caused activation of the DRG neurons. In conclusion, these results demonstrate a bidirectional cross-talk between cultured MMC and CGRP-IR DRG neurons in vitro. This indicates that such a communication may be the functional relevance for the close apposition between MMC and CGRP-IR nerve fibers in vivo. | lld:pubmed |
| pubmed-article:15016615 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15016615 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15016615 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15016615 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15016615 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15016615 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15016615 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15016615 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15016615 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:15016615 | pubmed:issn | 0193-1857 | lld:pubmed |
| pubmed-article:15016615 | pubmed:author | pubmed-author:BrownJ KJK | lld:pubmed |
| pubmed-article:15016615 | pubmed:author | pubmed-author:Van NassauwLL | lld:pubmed |
| pubmed-article:15016615 | pubmed:author | pubmed-author:De JonghEE | lld:pubmed |
| pubmed-article:15016615 | pubmed:author | pubmed-author:De LaetAA | lld:pubmed |
| pubmed-article:15016615 | pubmed:author | pubmed-author:TimmermansJ-P... | lld:pubmed |
| pubmed-article:15016615 | pubmed:author | pubmed-author:MillerH R PHR | lld:pubmed |
| pubmed-article:15016615 | pubmed:author | pubmed-author:KroeseA B AAB | lld:pubmed |
| pubmed-article:15016615 | pubmed:author | pubmed-author:van... | lld:pubmed |
| pubmed-article:15016615 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15016615 | pubmed:volume | 287 | lld:pubmed |
| pubmed-article:15016615 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15016615 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15016615 | pubmed:pagination | G178-91 | lld:pubmed |
| pubmed-article:15016615 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:15016615 | pubmed:meshHeading | pubmed-meshheading:15016615... | lld:pubmed |
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| pubmed-article:15016615 | pubmed:meshHeading | pubmed-meshheading:15016615... | lld:pubmed |
| pubmed-article:15016615 | pubmed:meshHeading | pubmed-meshheading:15016615... | lld:pubmed |
| pubmed-article:15016615 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15016615 | pubmed:articleTitle | In vitro activation of murine DRG neurons by CGRP-mediated mucosal mast cell degranulation. | lld:pubmed |
| pubmed-article:15016615 | pubmed:affiliation | Laboratory of Cell Biology and Histology, Department of Biomedical Sciences, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium. jean-pierre.timmermans@ua.ac.be | lld:pubmed |
| pubmed-article:15016615 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15016615 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:12310 | entrezgene:pubmed | pubmed-article:15016615 | lld:entrezgene |
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