| pubmed-article:15003383 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15003383 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:15003383 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:15003383 | lifeskim:mentions | umls-concept:C0013935 | lld:lifeskim |
| pubmed-article:15003383 | lifeskim:mentions | umls-concept:C1518263 | lld:lifeskim |
| pubmed-article:15003383 | lifeskim:mentions | umls-concept:C0006233 | lld:lifeskim |
| pubmed-article:15003383 | lifeskim:mentions | umls-concept:C1548779 | lld:lifeskim |
| pubmed-article:15003383 | lifeskim:mentions | umls-concept:C1947902 | lld:lifeskim |
| pubmed-article:15003383 | lifeskim:mentions | umls-concept:C2827499 | lld:lifeskim |
| pubmed-article:15003383 | lifeskim:mentions | umls-concept:C0598330 | lld:lifeskim |
| pubmed-article:15003383 | lifeskim:mentions | umls-concept:C1999230 | lld:lifeskim |
| pubmed-article:15003383 | lifeskim:mentions | umls-concept:C1511559 | lld:lifeskim |
| pubmed-article:15003383 | lifeskim:mentions | umls-concept:C1167624 | lld:lifeskim |
| pubmed-article:15003383 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:15003383 | pubmed:dateCreated | 2004-3-8 | lld:pubmed |
| pubmed-article:15003383 | pubmed:abstractText | I describe a method for providing cumulative label of bromodeoxyuridine (BrdU) to mouse embryos. Commercially available osmotic pumps, which release their contents at a steady rate, were implanted subcutaneously in the interscapular space of pregnant mice on embryonic day (E) 9.5-12.5. Survival times varied from 4h to 37 days. Tissues (embryonic and neonatal eyes and maternal intestine) were immunochemically labeled for BrdU and examined histologically. The first detectably labeled cells appeared 4-7h post-implantation (hpi) and all cycling cells were labeled for at least 7 days post-implantation (dpi). Retinal development appeared normal. Terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) stained retinas that had been exposed to BrdU showed no more apoptotic cells than those unexposed. I conclude that the maternally implanted osmotic pump successfully provides cumulative BrdU labeling in the mouse embryo. | lld:pubmed |
| pubmed-article:15003383 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15003383 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15003383 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15003383 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15003383 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15003383 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15003383 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15003383 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15003383 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:15003383 | pubmed:issn | 0165-0270 | lld:pubmed |
| pubmed-article:15003383 | pubmed:author | pubmed-author:FarahMohamed... | lld:pubmed |
| pubmed-article:15003383 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15003383 | pubmed:day | 30 | lld:pubmed |
| pubmed-article:15003383 | pubmed:volume | 134 | lld:pubmed |
| pubmed-article:15003383 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15003383 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15003383 | pubmed:pagination | 169-78 | lld:pubmed |
| pubmed-article:15003383 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:15003383 | pubmed:meshHeading | pubmed-meshheading:15003383... | lld:pubmed |
| pubmed-article:15003383 | pubmed:meshHeading | pubmed-meshheading:15003383... | lld:pubmed |
| pubmed-article:15003383 | pubmed:meshHeading | pubmed-meshheading:15003383... | lld:pubmed |
| pubmed-article:15003383 | pubmed:meshHeading | pubmed-meshheading:15003383... | lld:pubmed |
| pubmed-article:15003383 | pubmed:meshHeading | pubmed-meshheading:15003383... | lld:pubmed |
| pubmed-article:15003383 | pubmed:meshHeading | pubmed-meshheading:15003383... | lld:pubmed |
| pubmed-article:15003383 | pubmed:meshHeading | pubmed-meshheading:15003383... | lld:pubmed |
| pubmed-article:15003383 | pubmed:meshHeading | pubmed-meshheading:15003383... | lld:pubmed |
| pubmed-article:15003383 | pubmed:meshHeading | pubmed-meshheading:15003383... | lld:pubmed |
| pubmed-article:15003383 | pubmed:meshHeading | pubmed-meshheading:15003383... | lld:pubmed |
| pubmed-article:15003383 | pubmed:meshHeading | pubmed-meshheading:15003383... | lld:pubmed |
| pubmed-article:15003383 | pubmed:meshHeading | pubmed-meshheading:15003383... | lld:pubmed |
| pubmed-article:15003383 | pubmed:meshHeading | pubmed-meshheading:15003383... | lld:pubmed |
| pubmed-article:15003383 | pubmed:meshHeading | pubmed-meshheading:15003383... | lld:pubmed |
| pubmed-article:15003383 | pubmed:meshHeading | pubmed-meshheading:15003383... | lld:pubmed |
| pubmed-article:15003383 | pubmed:meshHeading | pubmed-meshheading:15003383... | lld:pubmed |
| pubmed-article:15003383 | pubmed:meshHeading | pubmed-meshheading:15003383... | lld:pubmed |
| pubmed-article:15003383 | pubmed:meshHeading | pubmed-meshheading:15003383... | lld:pubmed |
| pubmed-article:15003383 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15003383 | pubmed:articleTitle | Cumulative labeling of embryonic mouse neural retina with bromodeoxyuridine supplied by an osmotic minipump. | lld:pubmed |
| pubmed-article:15003383 | pubmed:affiliation | Neuroscience Program and Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI, USA. mfarah2@jhem.jhmi.edu | lld:pubmed |
| pubmed-article:15003383 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15003383 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:15003383 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:15003383 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
