| pubmed-article:14988155 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14988155 | lifeskim:mentions | umls-concept:C0019733 | lld:lifeskim |
| pubmed-article:14988155 | lifeskim:mentions | umls-concept:C0039195 | lld:lifeskim |
| pubmed-article:14988155 | lifeskim:mentions | umls-concept:C0027708 | lld:lifeskim |
| pubmed-article:14988155 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:14988155 | lifeskim:mentions | umls-concept:C0041361 | lld:lifeskim |
| pubmed-article:14988155 | lifeskim:mentions | umls-concept:C0596973 | lld:lifeskim |
| pubmed-article:14988155 | lifeskim:mentions | umls-concept:C0456387 | lld:lifeskim |
| pubmed-article:14988155 | lifeskim:mentions | umls-concept:C1524063 | lld:lifeskim |
| pubmed-article:14988155 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:14988155 | pubmed:dateCreated | 2004-6-4 | lld:pubmed |
| pubmed-article:14988155 | pubmed:abstractText | Recent studies have detected Wilms tumor antigen (WT1)-specific cytotoxic T lymphocytes (CTLs) in patients with acute myelogenous leukemia (AML) and chronic myelogenous leukemia (CML) and demonstrated that most of these CTLs were low avidity. Although HLA-mismatched donors can mount high-avidity CTLs against HLA-A2-presented peptides of WT1, a dominant anti-alloimmune response usually obscures detection of peptide-specific CTLs. Here we explored the feasibility of using recombinant HLA-A2 monomers containing single peptide epitopes as immunogens to generate peptide-specific CTLs from allogeneic donors. We demonstrate that the coating of HLA-A2(-) B lymphocytes with A2/peptide monomers provides a strong stimulus for autologous peptide-specific CTLs. After 3 to 5 rounds of stimulation a population of CD8(+) T cells binding A2/peptide tetramers is easily detectable by fluorescence-activated cell sorting analysis. Furthermore, sorted A2/WT1 tetramer-positive CTLs display strong cytotoxic activity against leukemia cells expressing WT1 endogenously but not against WT1(-) human tumor cells. Thus, HLA/peptide monomers may be useful to isolate peptide-specific donor lymphocytes for treatment of patients with leukemia after HLA-mismatched transplantation. | lld:pubmed |
| pubmed-article:14988155 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14988155 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14988155 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:14988155 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14988155 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14988155 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14988155 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:14988155 | pubmed:issn | 0006-4971 | lld:pubmed |
| pubmed-article:14988155 | pubmed:author | pubmed-author:GoulmyElsE | lld:pubmed |
| pubmed-article:14988155 | pubmed:author | pubmed-author:SavagePhilipP | lld:pubmed |
| pubmed-article:14988155 | pubmed:author | pubmed-author:CowburnPamP | lld:pubmed |
| pubmed-article:14988155 | pubmed:author | pubmed-author:ManStephenS | lld:pubmed |
| pubmed-article:14988155 | pubmed:author | pubmed-author:OggGrahamG | lld:pubmed |
| pubmed-article:14988155 | pubmed:author | pubmed-author:McMichaelAndr... | lld:pubmed |
| pubmed-article:14988155 | pubmed:author | pubmed-author:EpenetosAgame... | lld:pubmed |
| pubmed-article:14988155 | pubmed:author | pubmed-author:StaussHans... | lld:pubmed |
| pubmed-article:14988155 | pubmed:author | pubmed-author:GaoLiquanL | lld:pubmed |
| pubmed-article:14988155 | pubmed:author | pubmed-author:StevenNeilN | lld:pubmed |
| pubmed-article:14988155 | pubmed:author | pubmed-author:VentoKevinK | lld:pubmed |
| pubmed-article:14988155 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14988155 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:14988155 | pubmed:volume | 103 | lld:pubmed |
| pubmed-article:14988155 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14988155 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14988155 | pubmed:pagination | 4613-5 | lld:pubmed |
| pubmed-article:14988155 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:14988155 | pubmed:meshHeading | pubmed-meshheading:14988155... | lld:pubmed |
| pubmed-article:14988155 | pubmed:meshHeading | pubmed-meshheading:14988155... | lld:pubmed |
| pubmed-article:14988155 | pubmed:meshHeading | pubmed-meshheading:14988155... | lld:pubmed |
| pubmed-article:14988155 | pubmed:meshHeading | pubmed-meshheading:14988155... | lld:pubmed |
| pubmed-article:14988155 | pubmed:meshHeading | pubmed-meshheading:14988155... | lld:pubmed |
| pubmed-article:14988155 | pubmed:meshHeading | pubmed-meshheading:14988155... | lld:pubmed |
| pubmed-article:14988155 | pubmed:meshHeading | pubmed-meshheading:14988155... | lld:pubmed |
| pubmed-article:14988155 | pubmed:meshHeading | pubmed-meshheading:14988155... | lld:pubmed |
| pubmed-article:14988155 | pubmed:meshHeading | pubmed-meshheading:14988155... | lld:pubmed |
| pubmed-article:14988155 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:14988155 | pubmed:articleTitle | Use of B cell-bound HLA-A2 class I monomers to generate high-avidity, allo-restricted CTLs against the leukemia-associated protein Wilms tumor antigen. | lld:pubmed |
| pubmed-article:14988155 | pubmed:affiliation | Alexis Biotechnology Ltd., London, England, UK. | lld:pubmed |
| pubmed-article:14988155 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14988155 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:7490 | entrezgene:pubmed | pubmed-article:14988155 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:14988155 | lld:entrezgene |
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