| pubmed-article:1498594 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1498594 | lifeskim:mentions | umls-concept:C0242766 | lld:lifeskim |
| pubmed-article:1498594 | lifeskim:mentions | umls-concept:C0973457 | lld:lifeskim |
| pubmed-article:1498594 | lifeskim:mentions | umls-concept:C0031857 | lld:lifeskim |
| pubmed-article:1498594 | lifeskim:mentions | umls-concept:C0596988 | lld:lifeskim |
| pubmed-article:1498594 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:1498594 | pubmed:dateCreated | 1992-9-14 | lld:pubmed |
| pubmed-article:1498594 | pubmed:abstractText | A novel cDNA sequence homologous to a phytochrome B (phyB) gene that was isolated in a library from tobacco tissue has been used in an Escherichia coli expression system to raise anti-phytochrome B (anti-PHYB) polypeptide-specific monoclonal antibodies. The specificity of these antibodies has been tested by cross-reactivity against purified pea light-labile type 1 and light-stable type 2 phytochromes, with some antibodies reacting with the type 2 and none with the type 1 phytochromes. One such antibody, monoclonal mAT1, has been employed to analyze the phytochrome molecular species present in a photomorphogenic long hypocotyl (lh) mutant of cucumber. The results indicated that the mutant contains wild-type levels of the light-labile type 1 phytochrome polypeptide (PHYA), which has an apparent molecular mass of approximately 120 kD, but shows less than 1% (detection limit) of a light-stable polypeptide recognized by mAT1 in wild-type seedlings. This protein, not detectable in the lh mutant, has the properties of light-stable type 2 phytochrome, has an apparent molecular mass of 116 to 117 kD, and remains at constant levels under continuous low-fluence-rate red light. Therefore, we conclude that the lh mutant lacks at least one type 2 phytochrome-like polypeptide, most probably a phyB gene product. The correlation between the lack of this protein and the deficiency or absence of physiological responses to a light-stable phytochrome species in this mutant helps to identify the physiological roles played by the products of different subfamilies within the phytochrome gene family. | lld:pubmed |
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| pubmed-article:1498594 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1498594 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1498594 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1498594 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1498594 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:1498594 | pubmed:issn | 1040-4651 | lld:pubmed |
| pubmed-article:1498594 | pubmed:author | pubmed-author:FuruyaMM | lld:pubmed |
| pubmed-article:1498594 | pubmed:author | pubmed-author:TomizawaKK | lld:pubmed |
| pubmed-article:1498594 | pubmed:author | pubmed-author:DeanHH | lld:pubmed |
| pubmed-article:1498594 | pubmed:author | pubmed-author:KernRR | lld:pubmed |
| pubmed-article:1498594 | pubmed:author | pubmed-author:KendrickR ERE | lld:pubmed |
| pubmed-article:1498594 | pubmed:author | pubmed-author:NagataniAA | lld:pubmed |
| pubmed-article:1498594 | pubmed:author | pubmed-author:López-JuezEE | lld:pubmed |
| pubmed-article:1498594 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1498594 | pubmed:volume | 4 | lld:pubmed |
| pubmed-article:1498594 | pubmed:geneSymbol | phy | lld:pubmed |
| pubmed-article:1498594 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1498594 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1498594 | pubmed:pagination | 241-51 | lld:pubmed |
| pubmed-article:1498594 | pubmed:dateRevised | 2010-9-7 | lld:pubmed |
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| pubmed-article:1498594 | pubmed:meshHeading | pubmed-meshheading:1498594-... | lld:pubmed |
| pubmed-article:1498594 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1498594 | pubmed:articleTitle | The cucumber long hypocotyl mutant lacks a light-stable PHYB-like phytochrome. | lld:pubmed |
| pubmed-article:1498594 | pubmed:affiliation | Laboratory of Plant Biological Regulation, Frontier Research Program, RIKEN Institute, Saitama, Japan. | lld:pubmed |
| pubmed-article:1498594 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1498594 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1498594 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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