| pubmed-article:14980547 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14980547 | lifeskim:mentions | umls-concept:C0005767 | lld:lifeskim |
| pubmed-article:14980547 | lifeskim:mentions | umls-concept:C0368721 | lld:lifeskim |
| pubmed-article:14980547 | lifeskim:mentions | umls-concept:C0427620 | lld:lifeskim |
| pubmed-article:14980547 | lifeskim:mentions | umls-concept:C1260956 | lld:lifeskim |
| pubmed-article:14980547 | lifeskim:mentions | umls-concept:C0439836 | lld:lifeskim |
| pubmed-article:14980547 | lifeskim:mentions | umls-concept:C0175671 | lld:lifeskim |
| pubmed-article:14980547 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:14980547 | pubmed:dateCreated | 2004-2-24 | lld:pubmed |
| pubmed-article:14980547 | pubmed:abstractText | Accidental transfusion of ABO-incompatible red blood cells (RBCs) is a leading cause of fatal transfusion reactions. To prevent this and to create a universal blood supply, the idea of converting blood group A and B antigens to H using specific exo-glycosidases capable of removing the immunodominant sugar residues was pioneered by Goldstein and colleagues at the New York Blood Center in the early 1980s. Conversion of group B RBCs to O was initially carried out with alpha-galactosidase extracted from coffee beans. These enzyme-converted O (ECO) RBCs appeared to survive normally in all recipients independent of blood group. The clinical trials moved from small infusions to single RBC units and finally multiple and repeated transfusions. A successful phase II trial utilizing recombinant enzyme was reported by Kruskall and colleagues in 2000. Enzymatic conversion of group A RBCs has lagged behind due to lack of appropriate glycosidases and the more complex nature of A antigens. Identification of novel bacterial glycosidases with improved kinetic properties and specificities for the A and B antigens has greatly advanced the field. Conversion of group A RBCs can be achieved with improved glycosidases and the conversion conditions for both A and B antigens optimized to use more cost-efficient quantities of enzymes and gentler conditions including neutral pH and short incubation times at room temperature. Of the different strategies envisioned to create a universal blood supply, the ECO concept is the only one, for which human clinical trials have been performed. This paper discusses some biochemical and clinical aspects of this developing technology. | lld:pubmed |
| pubmed-article:14980547 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14980547 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14980547 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14980547 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14980547 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14980547 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14980547 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14980547 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14980547 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14980547 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14980547 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14980547 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14980547 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:14980547 | pubmed:issn | 1246-7820 | lld:pubmed |
| pubmed-article:14980547 | pubmed:author | pubmed-author:OlssonMartin... | lld:pubmed |
| pubmed-article:14980547 | pubmed:author | pubmed-author:ClausenHenrik... | lld:pubmed |
| pubmed-article:14980547 | pubmed:author | pubmed-author:KruskallMargo... | lld:pubmed |
| pubmed-article:14980547 | pubmed:author | pubmed-author:StroudMark... | lld:pubmed |
| pubmed-article:14980547 | pubmed:author | pubmed-author:HillCheryl... | lld:pubmed |
| pubmed-article:14980547 | pubmed:author | pubmed-author:de la... | lld:pubmed |
| pubmed-article:14980547 | pubmed:author | pubmed-author:LiuQiyong PQP | lld:pubmed |
| pubmed-article:14980547 | pubmed:author | pubmed-author:ValdinocciJea... | lld:pubmed |
| pubmed-article:14980547 | pubmed:author | pubmed-author:MoonStevenS | lld:pubmed |
| pubmed-article:14980547 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14980547 | pubmed:volume | 11 | lld:pubmed |
| pubmed-article:14980547 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14980547 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14980547 | pubmed:pagination | 33-9 | lld:pubmed |
| pubmed-article:14980547 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:14980547 | pubmed:meshHeading | pubmed-meshheading:14980547... | lld:pubmed |
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| pubmed-article:14980547 | pubmed:meshHeading | pubmed-meshheading:14980547... | lld:pubmed |
| pubmed-article:14980547 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:14980547 | pubmed:articleTitle | Universal red blood cells--enzymatic conversion of blood group A and B antigens. | lld:pubmed |
| pubmed-article:14980547 | pubmed:affiliation | Department of Transfusion Medicine, Institution of Laboratory Medicine, Lund University and Blood Center, University Hospital, 221 85 Lund, Sweden. Martin_L.Olsson@transfumed.lu.se | lld:pubmed |
| pubmed-article:14980547 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14980547 | pubmed:publicationType | Review | lld:pubmed |
