| pubmed-article:14980448 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14980448 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:14980448 | lifeskim:mentions | umls-concept:C0040648 | lld:lifeskim |
| pubmed-article:14980448 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:14980448 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:14980448 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:14980448 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:14980448 | lifeskim:mentions | umls-concept:C0243077 | lld:lifeskim |
| pubmed-article:14980448 | lifeskim:mentions | umls-concept:C1948059 | lld:lifeskim |
| pubmed-article:14980448 | lifeskim:mentions | umls-concept:C1720529 | lld:lifeskim |
| pubmed-article:14980448 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:14980448 | pubmed:dateCreated | 2004-2-24 | lld:pubmed |
| pubmed-article:14980448 | pubmed:abstractText | The truncated myc and max proteins, only containing basic regions and helix-loop-helix/zipper (b/HLH/Zip) regions were over-expressed in E. coli and used for the determination of the binding constant and of the inhibitory mechanism on myc-max (or max-max)-DNA complex formation. The association kinetic constants (k(1) and k(-1)) of truncated max-max or myc-max dimer and DNA were determined as k(1)=(1.7+/-0.6)x10(5) M(-1) s(-1), k(-1)=(3.4+/-1.2)x10(-2) s(-1) for max-max and DNA or k(1)=(2.1+/-0.7)x10(5) M(-1) s(-1), k(-1)=(3.2+/-1.4)x10(-2) s(-1) for myc-max and DNA. The equilibrium binding constant (K(1)) was determined using these kinetic parameters [K(XXD)=(7.8+/-2.6)x10(6) M(-1) for max-max and DNA or K(XYD)=(6.9+/-2.2)x10(6) M(-1) for myc-max and DNA]. The binding constants of myc-max or max-max dimer formation were K(XX)=(2.6+/-0.9)x10(5) M(-1) or K(XY)=(1.3+/-0.4)x10(4) M(-1), respectively. When truncated proteins were used, the max-max dimer formation was easier than the myc-max dimer formation, contrary to the physiologically determined case. This leads us to deduce that domains other than b/HLH/Zip are very important for the transcriptional regulatory activity in physiological conditions. The truncated myc and max proteins, which were expressed in E. coli and contained only b/HLH/Zip regions were also used for the screening of inhibitors of myc-max-DNA complex formation. A synthesized curcuminoid, 1,7-bis(4-methyl-3-nitrophenyl)-1,6-heptadiene-3,5-dione (curcuminoid 004), showed the most potent inhibition out of the synthesized curcuminoids, in competition with DNA. The dissociation constant of max-max dimer and the inhibitor was 9 microM, when investigated using in vitro expressed b/HLH/Zip dimer proteins. The curcuminoid 004 showed an inhibitory effect on the binding of myc-max protein to the E-box element in SNU16 cells, and suppressed the expression of myc target genes including ornithine decarboxylase (ODC), cdc25a and c-myc in myc over-expressed human stomach cancer cell line SNU16. | lld:pubmed |
| pubmed-article:14980448 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14980448 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14980448 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14980448 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14980448 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14980448 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14980448 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14980448 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14980448 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14980448 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14980448 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14980448 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14980448 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14980448 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14980448 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14980448 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:14980448 | pubmed:issn | 0006-3002 | lld:pubmed |
| pubmed-article:14980448 | pubmed:author | pubmed-author:YangChul-HakC... | lld:pubmed |
| pubmed-article:14980448 | pubmed:author | pubmed-author:HahmEun-Ryeon... | lld:pubmed |
| pubmed-article:14980448 | pubmed:author | pubmed-author:ParkChihoonC | lld:pubmed |
| pubmed-article:14980448 | pubmed:author | pubmed-author:ChungSunahS | lld:pubmed |
| pubmed-article:14980448 | pubmed:author | pubmed-author:ParkSeyeonS | lld:pubmed |
| pubmed-article:14980448 | pubmed:author | pubmed-author:KimKyung-MeeK... | lld:pubmed |
| pubmed-article:14980448 | pubmed:author | pubmed-author:JungKyung-Cha... | lld:pubmed |
| pubmed-article:14980448 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14980448 | pubmed:day | 24 | lld:pubmed |
| pubmed-article:14980448 | pubmed:volume | 1670 | lld:pubmed |
| pubmed-article:14980448 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14980448 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14980448 | pubmed:pagination | 217-28 | lld:pubmed |
| pubmed-article:14980448 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:14980448 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:14980448 | pubmed:articleTitle | Determination of binding constant of transcription factor myc-max/max-max and E-box DNA: the effect of inhibitors on the binding. | lld:pubmed |
| pubmed-article:14980448 | pubmed:affiliation | Samsung Medical Center, 50 Ilwon-Dong, Kangnam-Ku, Seoul 135-710, South Korea. | lld:pubmed |
| pubmed-article:14980448 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14980448 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14980448 | lld:pubmed |
