| pubmed-article:1497622 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1497622 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:1497622 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:1497622 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
| pubmed-article:1497622 | lifeskim:mentions | umls-concept:C0020852 | lld:lifeskim |
| pubmed-article:1497622 | lifeskim:mentions | umls-concept:C0023175 | lld:lifeskim |
| pubmed-article:1497622 | lifeskim:mentions | umls-concept:C0017982 | lld:lifeskim |
| pubmed-article:1497622 | lifeskim:mentions | umls-concept:C1705241 | lld:lifeskim |
| pubmed-article:1497622 | lifeskim:mentions | umls-concept:C1705242 | lld:lifeskim |
| pubmed-article:1497622 | lifeskim:mentions | umls-concept:C0591833 | lld:lifeskim |
| pubmed-article:1497622 | pubmed:dateCreated | 1992-9-10 | lld:pubmed |
| pubmed-article:1497622 | pubmed:abstractText | A monoclonal IgG-1 was produced by culture of a murine hybridoma (3.8.6) by three different methods, namely culture in ascites, in serum-free media and in serum-supplemented media. IgG-1 was purified to homogeneity (as judged by SDS/PAGE under reducing conditions) from each medium by ion-exchange chromatography and h.p.l.c. Protein A chromatography. Oligosaccharides were released from each IgG-1 preparation by hydrazinolysis and radiolabelled by reduction with alkaline sodium borotritide, and 'profile' analysis of the radiolabelled oligosaccharide alditols was performed by a combination of paper electrophoresis and gel-filtration chromatography. This analysis indicated clear and reproducible differences in the glycosylation patterns of the three IgG-1 preparations. Sequential exoglycosidase analysis of individual oligosaccharides derived from each IgG-1 preparation was used to define these differences. Ascites-derived material differed from serum-free-culture-derived material only with respect to the content of sialic acid. IgG-1 derived from culture in serum-containing media had an intermediate sialic acid content and a lower incidence of outer-arm galactosylation than the other two preparations. These differences in glycosylation could not be induced in any IgG-1 preparation by incubating purified IgG-1 with ascites or culture medium. It is concluded that the glycosylation pattern of a secreted monoclonal IgG is dependent on the culture method employed to obtain it. | lld:pubmed |
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| pubmed-article:1497622 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1497622 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1497622 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1497622 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1497622 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:1497622 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:1497622 | pubmed:author | pubmed-author:ParekhR BRB | lld:pubmed |
| pubmed-article:1497622 | pubmed:author | pubmed-author:PatelT PTP | lld:pubmed |
| pubmed-article:1497622 | pubmed:author | pubmed-author:PriorC PCP | lld:pubmed |
| pubmed-article:1497622 | pubmed:author | pubmed-author:MoelleringB... | lld:pubmed |
| pubmed-article:1497622 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1497622 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:1497622 | pubmed:volume | 285 ( Pt 3) | lld:pubmed |
| pubmed-article:1497622 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1497622 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1497622 | pubmed:pagination | 839-45 | lld:pubmed |
| pubmed-article:1497622 | pubmed:dateRevised | 2010-9-7 | lld:pubmed |
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| pubmed-article:1497622 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1497622 | pubmed:articleTitle | Different culture methods lead to differences in glycosylation of a murine IgG monoclonal antibody. | lld:pubmed |
| pubmed-article:1497622 | pubmed:affiliation | Oxford GlycoSystem Ltd., Abingdon, Oxen, U.K. | lld:pubmed |
| pubmed-article:1497622 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1497622 | pubmed:publicationType | Comparative Study | lld:pubmed |
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