| pubmed-article:14963033 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14963033 | lifeskim:mentions | umls-concept:C0001473 | lld:lifeskim |
| pubmed-article:14963033 | lifeskim:mentions | umls-concept:C0029904 | lld:lifeskim |
| pubmed-article:14963033 | lifeskim:mentions | umls-concept:C2611812 | lld:lifeskim |
| pubmed-article:14963033 | lifeskim:mentions | umls-concept:C1706853 | lld:lifeskim |
| pubmed-article:14963033 | lifeskim:mentions | umls-concept:C0597484 | lld:lifeskim |
| pubmed-article:14963033 | pubmed:issue | 17 | lld:pubmed |
| pubmed-article:14963033 | pubmed:dateCreated | 2004-4-19 | lld:pubmed |
| pubmed-article:14963033 | pubmed:abstractText | Based on the observation that the Na(+)/K(+)-ATPase alpha subunit contains two conserved caveolin-binding motifs, we hypothesized that clustering of the Na(+)/K(+)-ATPase and its partners in caveolae facilitates ouabain-activated signal transduction. Glutathione S-transferase pull-down assay showed that the Na(+)/K(+)-ATPase bound to the N terminus of caveolin-1. Significantly, ouabain regulated the interaction in a time- and dose-dependent manner and stimulated tyrosine phosphorylation of caveolin-1 in LLC-PK1 cells. When added to the isolated membrane fractions, ouabain increased tyrosine phosphorylation of proteins from the isolated caveolae but not other membrane fractions. Consistently, ouabain induced the formation of a Na(+)/K(+)-ATPase-Src-caveolin complex in the isolated caveolae preparations as it did in live cells. Finally, depletion of either cholesterol by methyl beta-cyclodextrin or caveolin-1 by siRNA significantly reduced the caveolar Na(+)/K(+)-ATPase and Src. Concomitantly, cholesterol depletion abolished ouabain-induced recruitment of Src to the Na(+)/K(+)-ATPase signaling complex. Like depletion of caveolin-1, it also blocked the effect of ouabain on ERKs, which was restored after cholesterol repletion. Clearly, the caveolar Na(+)/K(+)-ATPase represents the signaling pool of the pump that interacts with Src and transmits the ouabain signals. | lld:pubmed |
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| pubmed-article:14963033 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:14963033 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:14963033 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14963033 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:14963033 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:14963033 | pubmed:author | pubmed-author:RaskBB | lld:pubmed |
| pubmed-article:14963033 | pubmed:author | pubmed-author:HaasMichaelM | lld:pubmed |
| pubmed-article:14963033 | pubmed:author | pubmed-author:WangHaojieH | lld:pubmed |
| pubmed-article:14963033 | pubmed:author | pubmed-author:TianJiangJ | lld:pubmed |
| pubmed-article:14963033 | pubmed:author | pubmed-author:XieZijianZ | lld:pubmed |
| pubmed-article:14963033 | pubmed:author | pubmed-author:LiShengwenS | lld:pubmed |
| pubmed-article:14963033 | pubmed:author | pubmed-author:LiangManM | lld:pubmed |
| pubmed-article:14963033 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14963033 | pubmed:day | 23 | lld:pubmed |
| pubmed-article:14963033 | pubmed:volume | 279 | lld:pubmed |
| pubmed-article:14963033 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14963033 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14963033 | pubmed:pagination | 17250-9 | lld:pubmed |
| pubmed-article:14963033 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:14963033 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:14963033 | pubmed:articleTitle | Ouabain assembles signaling cascades through the caveolar Na+/K+-ATPase. | lld:pubmed |
| pubmed-article:14963033 | pubmed:affiliation | Department of Pharmacology and Medicine, Medical College of Ohio, Toledo, Ohio 43614, USA. | lld:pubmed |
| pubmed-article:14963033 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14963033 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:14963033 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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