| pubmed-article:1495986 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1495986 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:1495986 | lifeskim:mentions | umls-concept:C0004083 | lld:lifeskim |
| pubmed-article:1495986 | lifeskim:mentions | umls-concept:C0336791 | lld:lifeskim |
| pubmed-article:1495986 | lifeskim:mentions | umls-concept:C1999230 | lld:lifeskim |
| pubmed-article:1495986 | lifeskim:mentions | umls-concept:C0019873 | lld:lifeskim |
| pubmed-article:1495986 | lifeskim:mentions | umls-concept:C0205352 | lld:lifeskim |
| pubmed-article:1495986 | pubmed:issue | 15 | lld:pubmed |
| pubmed-article:1495986 | pubmed:dateCreated | 1992-9-4 | lld:pubmed |
| pubmed-article:1495986 | pubmed:abstractText | DNA-dependent and DNA-independent associations of DNA-binding proteins are important in transcriptional regulation. The analysis of DNA-independent associations frequently relies on assaying protein interaction in the absence of target DNA sequences. We have found that contaminating DNA in protein preparations can stabilize DNA-dependent associations that may appear DNA-independent. Three cellular proteins of 70, 85, and 110 kDa coimmunoprecipitated with the octamer motif-binding protein Oct-2 because of the presence of contaminating DNA in the cell extracts. In addition, heterodimer formation between Oct-1 (or Oct-2) and Pit-1 during protein-affinity chromatography was stabilized by the contaminating DNA. In both instances, these DNA-dependent protein associations were selectively inhibited by ethidium bromide in the precipitation reaction without any evident effect on DNA-independent protein associations. Thus, ethidium bromide may serve as a simple and general indicator of DNA-dependent and DNA-independent protein associations. | lld:pubmed |
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| pubmed-article:1495986 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:1495986 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1495986 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1495986 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:1495986 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:1495986 | pubmed:author | pubmed-author:DAYR CRC | lld:pubmed |
| pubmed-article:1495986 | pubmed:author | pubmed-author:HerrWW | lld:pubmed |
| pubmed-article:1495986 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1495986 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:1495986 | pubmed:volume | 89 | lld:pubmed |
| pubmed-article:1495986 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1495986 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1495986 | pubmed:pagination | 6958-62 | lld:pubmed |
| pubmed-article:1495986 | pubmed:dateRevised | 2010-9-7 | lld:pubmed |
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