| pubmed-article:1491699 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1491699 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:1491699 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:1491699 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:1491699 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:1491699 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:1491699 | lifeskim:mentions | umls-concept:C0553257 | lld:lifeskim |
| pubmed-article:1491699 | lifeskim:mentions | umls-concept:C0023607 | lld:lifeskim |
| pubmed-article:1491699 | lifeskim:mentions | umls-concept:C0018062 | lld:lifeskim |
| pubmed-article:1491699 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:1491699 | lifeskim:mentions | umls-concept:C0006556 | lld:lifeskim |
| pubmed-article:1491699 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:1491699 | lifeskim:mentions | umls-concept:C1709634 | lld:lifeskim |
| pubmed-article:1491699 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:1491699 | pubmed:dateCreated | 1993-2-26 | lld:pubmed |
| pubmed-article:1491699 | pubmed:abstractText | It is well established that the LH/CG receptor expressed in gonadal cells is an 85- to 92-kilodalton (kDa) glycoprotein. Additionally, however, a number of reports have noted the existence of other putative receptor species, but few attempts have been made to characterize these variant receptor species. A cell line [293L(wt1)] had previously been isolated which expresses large numbers of high affinity cell surface LH/CG receptors. Visualization of the LH/CG receptor species expressed in these cells and in rat luteal cells using ligand blots revealed 85- and 90-kDa LH/CG receptors, respectively, while immunoblots revealed another 68-kDa glycoprotein receptor in both cell types. The presence of both the 85- and 68-kDa receptor species was confirmed using immunoprecipitation and affinity purification of metabolically labeled 293L(wt1) cells. Enzymatic deglycosylations established that the 85-kDa receptor is a sialoprotein, while the 68-kDa species contains exposed high mannose residues. Protease digestion before LH/CG receptor immunoprecipitations localized the 85-kDa receptor on the plasma membrane, while the 68-kDa receptor was shown to be located intracellularly. Pulse-chase experiments were then used to positively establish that the 68-kDa receptor protein is actually a precursor of the 85-kDa LH/CG receptor species. | lld:pubmed |
| pubmed-article:1491699 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1491699 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1491699 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1491699 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1491699 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1491699 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1491699 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1491699 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1491699 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1491699 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1491699 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1491699 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:1491699 | pubmed:issn | 0888-8809 | lld:pubmed |
| pubmed-article:1491699 | pubmed:author | pubmed-author:AscoliMM | lld:pubmed |
| pubmed-article:1491699 | pubmed:author | pubmed-author:Sánchez-Yagüe... | lld:pubmed |
| pubmed-article:1491699 | pubmed:author | pubmed-author:HipkinR WRW | lld:pubmed |
| pubmed-article:1491699 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1491699 | pubmed:volume | 6 | lld:pubmed |
| pubmed-article:1491699 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1491699 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1491699 | pubmed:pagination | 2210-8 | lld:pubmed |
| pubmed-article:1491699 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:1491699 | pubmed:meshHeading | pubmed-meshheading:1491699-... | lld:pubmed |
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| pubmed-article:1491699 | pubmed:meshHeading | pubmed-meshheading:1491699-... | lld:pubmed |
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| pubmed-article:1491699 | pubmed:meshHeading | pubmed-meshheading:1491699-... | lld:pubmed |
| pubmed-article:1491699 | pubmed:meshHeading | pubmed-meshheading:1491699-... | lld:pubmed |
| pubmed-article:1491699 | pubmed:meshHeading | pubmed-meshheading:1491699-... | lld:pubmed |
| pubmed-article:1491699 | pubmed:meshHeading | pubmed-meshheading:1491699-... | lld:pubmed |
| pubmed-article:1491699 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1491699 | pubmed:articleTitle | Identification and characterization of a luteinizing hormone/chorionic gonadotropin (LH/CG) receptor precursor in a human kidney cell line stably transfected with the rat luteal LH/CG receptor complementary DNA. | lld:pubmed |
| pubmed-article:1491699 | pubmed:affiliation | Department of Pharmacology, University of Iowa College of Medicine, Iowa City 52242. | lld:pubmed |
| pubmed-article:1491699 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1491699 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1491699 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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