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pubmed-article:14767825pubmed:abstractTextWe compared the rates of detection of respiratory viruses by reverse-transcription polymerase chain reaction (RT-PCR) and by conventional viral culture in 668 combined nasal and throat samples from a prospective, multicenter, population-based study of acute respiratory tract infections among hospitalized children aged <5 years. RT-PCR increased the yield of viral identification by 2-fold, compared with that of culture alone. The increased sensitivity of viral detection by RT-PCR will yield better estimates of the population burden of viral respiratory infections.lld:pubmed
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pubmed-article:14767825pubmed:articleTitleSuperiority of reverse-transcription polymerase chain reaction to conventional viral culture in the diagnosis of acute respiratory tract infections in children.lld:pubmed
pubmed-article:14767825pubmed:affiliationDepartment of Pediatrics and Strong Children's Research Center, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA. geoff_weinberg@urmc.rochester.edulld:pubmed
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