| pubmed-article:14733492 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14733492 | lifeskim:mentions | umls-concept:C0019236 | lld:lifeskim |
| pubmed-article:14733492 | lifeskim:mentions | umls-concept:C0014441 | lld:lifeskim |
| pubmed-article:14733492 | lifeskim:mentions | umls-concept:C0066691 | lld:lifeskim |
| pubmed-article:14733492 | lifeskim:mentions | umls-concept:C0332324 | lld:lifeskim |
| pubmed-article:14733492 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:14733492 | pubmed:dateCreated | 2004-1-21 | lld:pubmed |
| pubmed-article:14733492 | pubmed:abstractText | This paper reports on the generation of monoclonal antibodies and the development of a new enzyme-linked immunosorbent assay (ELISA) for the detection of molinate (S-ethyl hexahydroazepine-1-carbothioate). Hybridoma cells were generated using spleen and lymph node cells from a mouse immunized with S-2-carboxyethyl hexahydroazepine-1-carbothioate conjugated to keyhole limpet hemocyanin. After screening with a competitive ELISA, two monoclonal antibodies, mAbs 16C11 and 14D7, with IC(50) values of 82 +/- 2 and 173 +/- 8 ng/mL, respectively, were selected. MAb 16C11 can detect molinate concentrations of 1 ng/mL with no cross-reactivity to any other thiocarbamate pesticides; however, it was susceptible to the presence of organic solvents and to variation in buffer ionic strength. MAb 14D7 tolerated concentrations up to 5% of propylene glycol and 12.5% of acetonitrile in the assay buffer. The sensitivity of mAb 14D7 was further improved by decreasing the amount of coating antigen in the ELISA; the final inhibition assay showed an IC(50) of 69.2 +/- 1.4 ng/mL. In summary, mAb14D7 provided a more sensitive and robust assay, as compared with previous polyclonal antibody-based assays, with the additional advantage of being based upon a consistent and unlimited source of a defined reagent. | lld:pubmed |
| pubmed-article:14733492 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14733492 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14733492 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14733492 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14733492 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14733492 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14733492 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14733492 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14733492 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14733492 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14733492 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14733492 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14733492 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:14733492 | pubmed:issn | 0021-8561 | lld:pubmed |
| pubmed-article:14733492 | pubmed:author | pubmed-author:HammockBruce... | lld:pubmed |
| pubmed-article:14733492 | pubmed:author | pubmed-author:RufoCaterinaC | lld:pubmed |
| pubmed-article:14733492 | pubmed:author | pubmed-author:LastJerold... | lld:pubmed |
| pubmed-article:14733492 | pubmed:author | pubmed-author:GeeShirley... | lld:pubmed |
| pubmed-article:14733492 | pubmed:author | pubmed-author:González-Sapi... | lld:pubmed |
| pubmed-article:14733492 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14733492 | pubmed:day | 28 | lld:pubmed |
| pubmed-article:14733492 | pubmed:volume | 52 | lld:pubmed |
| pubmed-article:14733492 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14733492 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14733492 | pubmed:pagination | 182-7 | lld:pubmed |
| pubmed-article:14733492 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:14733492 | pubmed:meshHeading | pubmed-meshheading:14733492... | lld:pubmed |
| pubmed-article:14733492 | pubmed:meshHeading | pubmed-meshheading:14733492... | lld:pubmed |
| pubmed-article:14733492 | pubmed:meshHeading | pubmed-meshheading:14733492... | lld:pubmed |
| pubmed-article:14733492 | pubmed:meshHeading | pubmed-meshheading:14733492... | lld:pubmed |
| pubmed-article:14733492 | pubmed:meshHeading | pubmed-meshheading:14733492... | lld:pubmed |
| pubmed-article:14733492 | pubmed:meshHeading | pubmed-meshheading:14733492... | lld:pubmed |
| pubmed-article:14733492 | pubmed:meshHeading | pubmed-meshheading:14733492... | lld:pubmed |
| pubmed-article:14733492 | pubmed:meshHeading | pubmed-meshheading:14733492... | lld:pubmed |
| pubmed-article:14733492 | pubmed:meshHeading | pubmed-meshheading:14733492... | lld:pubmed |
| pubmed-article:14733492 | pubmed:meshHeading | pubmed-meshheading:14733492... | lld:pubmed |
| pubmed-article:14733492 | pubmed:meshHeading | pubmed-meshheading:14733492... | lld:pubmed |
| pubmed-article:14733492 | pubmed:meshHeading | pubmed-meshheading:14733492... | lld:pubmed |
| pubmed-article:14733492 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:14733492 | pubmed:articleTitle | Robust and sensitive monoclonal enzyme-linked immunosorbent assay for the herbicide molinate. | lld:pubmed |
| pubmed-article:14733492 | pubmed:affiliation | Cátedra de Inmunología, Facultad de Química, Universidad de la República, Instituto de Higiene, Av. A. Navarro 3051, 11600 Montevideo, Uruguay. | lld:pubmed |
| pubmed-article:14733492 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14733492 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14733492 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14733492 | lld:pubmed |
