| pubmed-article:14695827 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14695827 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:14695827 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
| pubmed-article:14695827 | lifeskim:mentions | umls-concept:C1148600 | lld:lifeskim |
| pubmed-article:14695827 | lifeskim:mentions | umls-concept:C0870071 | lld:lifeskim |
| pubmed-article:14695827 | lifeskim:mentions | umls-concept:C0887819 | lld:lifeskim |
| pubmed-article:14695827 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:14695827 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:14695827 | lifeskim:mentions | umls-concept:C0450363 | lld:lifeskim |
| pubmed-article:14695827 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:14695827 | pubmed:dateCreated | 2003-12-29 | lld:pubmed |
| pubmed-article:14695827 | pubmed:abstractText | Human monoclonal antibodies (mAbs) designed for immunotherapy have a high potential for avoiding the complications that may result from human immune system responses to the introduction of nonhuman mAbs into patients. This study presents a characterization of cocaine/antibody interactions that determine the binding properties of the novel human sequence mAb 2E2 using three-dimensional quantitative structure-activity relationship (3D-QSAR) methodology. We have experimentally determined the binding affinities of mAb 2E2 for cocaine and 38 cocaine analogues. The K(d) of mAb 2E2 for cocaine was 4 nM, indicating a high affinity. Also, mAb 2E2 displayed good cocaine specificity, as reflected in its 10-, 1500-, and 25000-fold lower binding affinities for the three physiologically relevant cocaine metabolites benzoylecgonine, ecgonine methyl ester, and ecgonine, respectively. 3D-QSAR models of cocaine binding were developed by comparative molecular similarity index analysis (CoMSIA). A model of high statistical quality was generated showing that cocaine binds to mAb 2E2 in a sterically restricted binding site that leaves the methyl group attached to the ring nitrogen of cocaine solvent-exposed. The methyl ester group of cocaine appears to engage in attractive van der Waals interactions with mAb 2E2, whereas the phenyl group contributes to the binding primarily via hydrophobic interactions. The model further indicated that an increase in partial positive charge near the nitrogen proton and methyl ester carbonyl group enhances binding affinity and that the ester oxygen likely forms an intermolecular hydrogen bond with mAb 2E2. Overall, the cocaine binding properties of mAb 2E2 support its clinical potential for development as a treatment of cocaine overdose and addiction. | lld:pubmed |
| pubmed-article:14695827 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14695827 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14695827 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14695827 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14695827 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14695827 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14695827 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14695827 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:14695827 | pubmed:issn | 0022-2623 | lld:pubmed |
| pubmed-article:14695827 | pubmed:author | pubmed-author:FarrCarol DCD | lld:pubmed |
| pubmed-article:14695827 | pubmed:author | pubmed-author:TabetMichael... | lld:pubmed |
| pubmed-article:14695827 | pubmed:author | pubmed-author:NormanAndrew... | lld:pubmed |
| pubmed-article:14695827 | pubmed:author | pubmed-author:PaulaStefanS | lld:pubmed |
| pubmed-article:14695827 | pubmed:author | pubmed-author:BallW... | lld:pubmed |
| pubmed-article:14695827 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14695827 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:14695827 | pubmed:volume | 47 | lld:pubmed |
| pubmed-article:14695827 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14695827 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14695827 | pubmed:pagination | 133-42 | lld:pubmed |
| pubmed-article:14695827 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:14695827 | pubmed:meshHeading | pubmed-meshheading:14695827... | lld:pubmed |
| pubmed-article:14695827 | pubmed:meshHeading | pubmed-meshheading:14695827... | lld:pubmed |
| pubmed-article:14695827 | pubmed:meshHeading | pubmed-meshheading:14695827... | lld:pubmed |
| pubmed-article:14695827 | pubmed:meshHeading | pubmed-meshheading:14695827... | lld:pubmed |
| pubmed-article:14695827 | pubmed:meshHeading | pubmed-meshheading:14695827... | lld:pubmed |
| pubmed-article:14695827 | pubmed:meshHeading | pubmed-meshheading:14695827... | lld:pubmed |
| pubmed-article:14695827 | pubmed:meshHeading | pubmed-meshheading:14695827... | lld:pubmed |
| pubmed-article:14695827 | pubmed:meshHeading | pubmed-meshheading:14695827... | lld:pubmed |
| pubmed-article:14695827 | pubmed:meshHeading | pubmed-meshheading:14695827... | lld:pubmed |
| pubmed-article:14695827 | pubmed:meshHeading | pubmed-meshheading:14695827... | lld:pubmed |
| pubmed-article:14695827 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:14695827 | pubmed:articleTitle | Three-dimensional quantitative structure-activity relationship modeling of cocaine binding by a novel human monoclonal antibody. | lld:pubmed |
| pubmed-article:14695827 | pubmed:affiliation | Department of Pharmacology and Cell Biophysics, College of Medicine, University of Cincinnati, Cincinnati, Ohio 45267-0575, USA. | lld:pubmed |
| pubmed-article:14695827 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14695827 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | http://linkedlifedata.com/r... | pubmed-article:14695827 | lld:chembl |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14695827 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14695827 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14695827 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14695827 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14695827 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14695827 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14695827 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14695827 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14695827 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14695827 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14695827 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14695827 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14695827 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14695827 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14695827 | lld:pubmed |
