| pubmed-article:14677134 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14677134 | lifeskim:mentions | umls-concept:C0011923 | lld:lifeskim |
| pubmed-article:14677134 | lifeskim:mentions | umls-concept:C0016315 | lld:lifeskim |
| pubmed-article:14677134 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:14677134 | pubmed:dateCreated | 2003-12-16 | lld:pubmed |
| pubmed-article:14677134 | pubmed:abstractText | We present a time-correlated single photon counting (TCPSC) technique that allows time-resolved multi-wavelength imaging in conjunction with a laser scanning microscope and a pulsed excitation source. The technique is based on a four-dimensional histogramming process that records the photon density over the time of the fluorescence decay, the x-y coordinates of the scanning area, and the wavelength. The histogramming process avoids any time gating or wavelength scanning and, therefore, yields a near-perfect counting efficiency. The time resolution is limited only by the transit time spread of the detector. The technique can be used with almost any confocal or two-photon laser scanning microscope and works at any scanning rate. We demonstrate the application to samples stained with several dyes and to CFP-YFP FRET. | lld:pubmed |
| pubmed-article:14677134 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14677134 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14677134 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14677134 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14677134 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:14677134 | pubmed:issn | 1059-910X | lld:pubmed |
| pubmed-article:14677134 | pubmed:author | pubmed-author:KönigKK | lld:pubmed |
| pubmed-article:14677134 | pubmed:author | pubmed-author:BeckerWW | lld:pubmed |
| pubmed-article:14677134 | pubmed:author | pubmed-author:BergmannAA | lld:pubmed |
| pubmed-article:14677134 | pubmed:author | pubmed-author:BenndorfKK | lld:pubmed |
| pubmed-article:14677134 | pubmed:author | pubmed-author:BiskupCC | lld:pubmed |
| pubmed-article:14677134 | pubmed:author | pubmed-author:HinkM AMA | lld:pubmed |
| pubmed-article:14677134 | pubmed:copyrightInfo | Copyright 2003 Wiley-Liss, Inc. | lld:pubmed |
| pubmed-article:14677134 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14677134 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:14677134 | pubmed:volume | 63 | lld:pubmed |
| pubmed-article:14677134 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14677134 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14677134 | pubmed:pagination | 58-66 | lld:pubmed |
| pubmed-article:14677134 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
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| pubmed-article:14677134 | pubmed:meshHeading | pubmed-meshheading:14677134... | lld:pubmed |
| pubmed-article:14677134 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:14677134 | pubmed:articleTitle | Fluorescence lifetime imaging by time-correlated single-photon counting. | lld:pubmed |
| pubmed-article:14677134 | pubmed:affiliation | Becker & Hickl GmbH, D-12277 Berlin, Germany. becker@becker-hickl.com | lld:pubmed |
| pubmed-article:14677134 | pubmed:publicationType | Journal Article | lld:pubmed |
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