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pubmed-article:1466358pubmed:abstractTextSince transforming growth factor beta (TGF beta) has been implicated as an important mediator of pulmonary fibrosis, we measured TGF beta protein and gene expression in alveolar epithelial lining fluid (ELF) of fibrotic scleroderma lungs sampled by bronchoalveolar lavage (BAL). TGF beta protein was qualitatively examined by Western blot analysis, and quantitatively by radioreceptor assays. Gene expression was evaluated in BAL mononuclear cells by Northern blot analysis with quantification of relative gene expression by densitometric analysis of the autoradiograms.lld:pubmed
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pubmed-article:1466358pubmed:articleTitleTransforming growth factor beta within fibrotic scleroderma lungs.lld:pubmed
pubmed-article:1466358pubmed:affiliationDepartment of Medicine, University of Alabama School of Medicine, Birmingham 35294.lld:pubmed
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