| pubmed-article:14653316 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14653316 | lifeskim:mentions | umls-concept:C1486096 | lld:lifeskim |
| pubmed-article:14653316 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:14653316 | lifeskim:mentions | umls-concept:C0017547 | lld:lifeskim |
| pubmed-article:14653316 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:14653316 | lifeskim:mentions | umls-concept:C0599161 | lld:lifeskim |
| pubmed-article:14653316 | lifeskim:mentions | umls-concept:C0016710 | lld:lifeskim |
| pubmed-article:14653316 | lifeskim:mentions | umls-concept:C0162788 | lld:lifeskim |
| pubmed-article:14653316 | lifeskim:mentions | umls-concept:C0917787 | lld:lifeskim |
| pubmed-article:14653316 | lifeskim:mentions | umls-concept:C0450254 | lld:lifeskim |
| pubmed-article:14653316 | lifeskim:mentions | umls-concept:C1033922 | lld:lifeskim |
| pubmed-article:14653316 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:14653316 | pubmed:dateCreated | 2003-12-4 | lld:pubmed |
| pubmed-article:14653316 | pubmed:abstractText | The availability of specific and rapid detection methods is essential for monitoring the health status of farmed species, particularly in viral diseases as in this case early diagnosis is a critical factor in containing disease outbreaks. Three complementary genome-based methods were developed for the detection of Macrobrachium rosenbergii nodavirus (MrNV), i.e. dot-blot hybridization, in situ hybridization and reverse transcriptase-polymerase chain reaction (RT-PCR). Detection limits were established for dot-blot hybridization and RT-PCR and are c. 7 fg and 8 pg of viral RNA, respectively. In situ hybridization indicated that infection was confined to the striated muscle tissue. As a result of its sensitivity, RT-PCR can be used for in-depth investigations to examine the extent of the viral infection and establish the onset of infection in hatcheries. The application of RT-PCR on samples collected from prawn farms in China showed the possible use of this method in routine health monitoring. | lld:pubmed |
| pubmed-article:14653316 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14653316 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14653316 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14653316 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14653316 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14653316 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:14653316 | pubmed:issn | 0140-7775 | lld:pubmed |
| pubmed-article:14653316 | pubmed:author | pubmed-author:DurandSS | lld:pubmed |
| pubmed-article:14653316 | pubmed:author | pubmed-author:BonamiJ RJR | lld:pubmed |
| pubmed-article:14653316 | pubmed:author | pubmed-author:Sri WidadaJJ | lld:pubmed |
| pubmed-article:14653316 | pubmed:author | pubmed-author:ShoTT | lld:pubmed |
| pubmed-article:14653316 | pubmed:author | pubmed-author:RichardVV | lld:pubmed |
| pubmed-article:14653316 | pubmed:author | pubmed-author:QianDD | lld:pubmed |
| pubmed-article:14653316 | pubmed:author | pubmed-author:CambournacII | lld:pubmed |
| pubmed-article:14653316 | pubmed:author | pubmed-author:DejongheEE | lld:pubmed |
| pubmed-article:14653316 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14653316 | pubmed:volume | 26 | lld:pubmed |
| pubmed-article:14653316 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14653316 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14653316 | pubmed:pagination | 583-90 | lld:pubmed |
| pubmed-article:14653316 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:14653316 | pubmed:meshHeading | pubmed-meshheading:14653316... | lld:pubmed |
| pubmed-article:14653316 | pubmed:meshHeading | pubmed-meshheading:14653316... | lld:pubmed |
| pubmed-article:14653316 | pubmed:meshHeading | pubmed-meshheading:14653316... | lld:pubmed |
| pubmed-article:14653316 | pubmed:meshHeading | pubmed-meshheading:14653316... | lld:pubmed |
| pubmed-article:14653316 | pubmed:meshHeading | pubmed-meshheading:14653316... | lld:pubmed |
| pubmed-article:14653316 | pubmed:meshHeading | pubmed-meshheading:14653316... | lld:pubmed |
| pubmed-article:14653316 | pubmed:meshHeading | pubmed-meshheading:14653316... | lld:pubmed |
| pubmed-article:14653316 | pubmed:meshHeading | pubmed-meshheading:14653316... | lld:pubmed |
| pubmed-article:14653316 | pubmed:meshHeading | pubmed-meshheading:14653316... | lld:pubmed |
| pubmed-article:14653316 | pubmed:meshHeading | pubmed-meshheading:14653316... | lld:pubmed |
| pubmed-article:14653316 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:14653316 | pubmed:articleTitle | Genome-based detection methods of Macrobrachium rosenbergii nodavirus, a pathogen of the giant freshwater prawn, Macrobrachium rosenbergii dot-blot, in situ hybridization and RT-PCR. | lld:pubmed |
| pubmed-article:14653316 | pubmed:affiliation | Pathogens and Immunity, CNRS, Université Montpellier II, Place Eugène Bataillon, Montpellier, France. widada@crit.univ-montp2.fr | lld:pubmed |
| pubmed-article:14653316 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14653316 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14653316 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14653316 | lld:pubmed |
