| pubmed-article:1464601 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1464601 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:1464601 | lifeskim:mentions | umls-concept:C0596233 | lld:lifeskim |
| pubmed-article:1464601 | pubmed:issue | 36 | lld:pubmed |
| pubmed-article:1464601 | pubmed:dateCreated | 1993-1-21 | lld:pubmed |
| pubmed-article:1464601 | pubmed:abstractText | Mechanisms controlling Ca2+ fluxes through the plasma membrane of lymphocytes have been characterized in a human T-cell clone and in the Jurkat T-cell line. Due to endogenous buffers, about 1/125 of the Ca2+ ions that enter the cell are free. Ca2+ fluxes were estimated from the variations in intracellular Ca2+ concentration ([Ca2+]i) elicited by concentration jumps in extracellular Ca2+ ([Ca2+]o). Thapsigargin was used to inhibit Ca2+ uptake into intracellular stores and to stimulate Ca2+ entry. Ca2+ extrusion was strictly due to the activity of plasma membrane Ca(2+)-ATPases since there was no detectable Na+/Ca2+ exchange activity in these cells. The rate of Ca2+ extrusion was mainly influenced by [Ca2+]i and less by [Ca2+]o but was insensitive to cell depolarization. In depolarized cells, thapsigargin-induced Ca2+ influx was reduced to 10% of the value measured in normally polarized cells, suggesting that depolarization not only reduces the electrochemical gradient for Ca2+ ions, but also inhibits Ca2+ permeation. When Ca2+ ions enter the cell, they bind to a site inside the channel, with a Kd of 3.3 mM. Stimulation of clonal T-cells with low concentrations of either anti-CD3 antibodies or thapsigargin elicited Ca2+ oscillations. Both the amplitude and the frequency of CD3-induced Ca2+ oscillations were sensitive to [Ca2+]o. These oscillations were immediately interrupted when extracellular Ca2+ was removed. The properties of Ca2+ oscillations in T lymphocytes suggest that they are mainly due to variations of Ca2+ influx, modulated by variations in [Ca2+]i. | lld:pubmed |
| pubmed-article:1464601 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1464601 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1464601 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1464601 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1464601 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:1464601 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:1464601 | pubmed:author | pubmed-author:TrautmannAA | lld:pubmed |
| pubmed-article:1464601 | pubmed:author | pubmed-author:BismuthGG | lld:pubmed |
| pubmed-article:1464601 | pubmed:author | pubmed-author:DonnadieuEE | lld:pubmed |
| pubmed-article:1464601 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1464601 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:1464601 | pubmed:volume | 267 | lld:pubmed |
| pubmed-article:1464601 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1464601 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1464601 | pubmed:pagination | 25864-72 | lld:pubmed |
| pubmed-article:1464601 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:1464601 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1464601 | pubmed:articleTitle | Calcium fluxes in T lymphocytes. | lld:pubmed |
| pubmed-article:1464601 | pubmed:affiliation | Laboratoire de Neurobiologie, Centre National de la Recherche Scientifique (CNRS) URA 295, Ecole Normale Supérieure, Paris, France. | lld:pubmed |
| pubmed-article:1464601 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1464601 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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