| pubmed-article:14645272 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14645272 | lifeskim:mentions | umls-concept:C0033809 | lld:lifeskim |
| pubmed-article:14645272 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:14645272 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:14645272 | lifeskim:mentions | umls-concept:C1514562 | lld:lifeskim |
| pubmed-article:14645272 | lifeskim:mentions | umls-concept:C1883221 | lld:lifeskim |
| pubmed-article:14645272 | lifeskim:mentions | umls-concept:C1704735 | lld:lifeskim |
| pubmed-article:14645272 | lifeskim:mentions | umls-concept:C1883204 | lld:lifeskim |
| pubmed-article:14645272 | lifeskim:mentions | umls-concept:C1880389 | lld:lifeskim |
| pubmed-article:14645272 | pubmed:issue | 24 | lld:pubmed |
| pubmed-article:14645272 | pubmed:dateCreated | 2003-12-3 | lld:pubmed |
| pubmed-article:14645272 | pubmed:abstractText | The RhlR transcriptional regulator of Pseudomonas aeruginosa, along with its cognate autoinducer, N-butyryl homoserine lactone (C(4)-HSL), regulates gene expression in response to cell density. With an Escherichia coli LexA-based protein interaction system, we demonstrated that RhlR multimerized and that the degree of multimerization was dependent on the C(4)-HSL concentration. Studies with an E. coli lasB::lacZ lysogen demonstrated that RhlR multimerization was necessary for it to function as a transcriptional activator. Deletion analysis of RhlR indicated that the N-terminal domain of the protein is necessary for C(4)-HSL binding. Single amino acid substitutions in the C-terminal domain of RhlR generated mutant RhlR proteins that had the ability to bind C(4)-HSL and multimerize but were unable to activate lasB expression, demonstrating that the C-terminal domain is important for target gene activation. Single amino acid substitutions in both the N-terminal and C-terminal domains of RhlR demonstrated that both domains possess residues involved in multimerization. RhlR with a C-terminal deletion and an RhlR site-specific mutant form that possessed multimerization but not transcriptional activation capabilities were able to inhibit the ability of wild-type RhlR to activate rhlA expression in P. aeruginosa. We conclude that C(4)-HSL binding is necessary for RhlR multimerization and that RhlR functions as a multimer in P. aeruginosa. | lld:pubmed |
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| pubmed-article:14645272 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14645272 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14645272 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:14645272 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14645272 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:14645272 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:14645272 | pubmed:author | pubmed-author:PatelHetalH | lld:pubmed |
| pubmed-article:14645272 | pubmed:author | pubmed-author:IglewskiBarba... | lld:pubmed |
| pubmed-article:14645272 | pubmed:author | pubmed-author:WagnerVictori... | lld:pubmed |
| pubmed-article:14645272 | pubmed:author | pubmed-author:LambJanet RJR | lld:pubmed |
| pubmed-article:14645272 | pubmed:author | pubmed-author:MontminyTimot... | lld:pubmed |
| pubmed-article:14645272 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14645272 | pubmed:volume | 185 | lld:pubmed |
| pubmed-article:14645272 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14645272 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14645272 | pubmed:pagination | 7129-39 | lld:pubmed |
| pubmed-article:14645272 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:14645272 | pubmed:meshHeading | pubmed-meshheading:14645272... | lld:pubmed |
| pubmed-article:14645272 | pubmed:meshHeading | pubmed-meshheading:14645272... | lld:pubmed |
| pubmed-article:14645272 | pubmed:meshHeading | pubmed-meshheading:14645272... | lld:pubmed |
| pubmed-article:14645272 | pubmed:meshHeading | pubmed-meshheading:14645272... | lld:pubmed |
| pubmed-article:14645272 | pubmed:meshHeading | pubmed-meshheading:14645272... | lld:pubmed |
| pubmed-article:14645272 | pubmed:meshHeading | pubmed-meshheading:14645272... | lld:pubmed |
| pubmed-article:14645272 | pubmed:meshHeading | pubmed-meshheading:14645272... | lld:pubmed |
| pubmed-article:14645272 | pubmed:meshHeading | pubmed-meshheading:14645272... | lld:pubmed |
| pubmed-article:14645272 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:14645272 | pubmed:articleTitle | Functional domains of the RhlR transcriptional regulator of Pseudomonas aeruginosa. | lld:pubmed |
| pubmed-article:14645272 | pubmed:affiliation | University of Rochester, Rochester, New York 14642, USA. | lld:pubmed |
| pubmed-article:14645272 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14645272 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:14645272 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14645272 | lld:pubmed |
