| pubmed-article:14630694 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14630694 | lifeskim:mentions | umls-concept:C0008109 | lld:lifeskim |
| pubmed-article:14630694 | lifeskim:mentions | umls-concept:C0919528 | lld:lifeskim |
| pubmed-article:14630694 | lifeskim:mentions | umls-concept:C0015133 | lld:lifeskim |
| pubmed-article:14630694 | lifeskim:mentions | umls-concept:C0178648 | lld:lifeskim |
| pubmed-article:14630694 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:14630694 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:14630694 | pubmed:dateCreated | 2004-1-13 | lld:pubmed |
| pubmed-article:14630694 | pubmed:abstractText | MLL gene fusions are the hallmark of more than 70% of therapy-related leukemias (t-ML) associated with topoisomerase II inhibitors (e.g., etoposide) and cause leukemia in murine transgenic models. To determine whether Mll genomic fusions can occur after exposure to topoisomerase II inhibitors, we developed a long-distance inverse PCR DNA-based assay for chimeric Mll fusions in mouse embryonic stem cells. We detected Mll fusions at a higher frequency following 100 microM etoposide for 8 h (16x10(-6) cell(-1)) than in no-drug controls (1.0x10(-6) cell(-1), P=0.0002) or after treatment with a comparably cytotoxic exposure to the antimicrotubule drug vincristine (1.0x10(-6) cell(-1), P=0.0047). The fusion points in Mll chimeric products induced by etoposide were localized to a 1.5 kb region between exons 9 and 11, analogous to the MLL breakpoint cluster region in human leukemia. All 49 Mll fusion partners analyzed matched known genomic murine sequences, with 40 (82%) matching annotated genes covering eighteen murine autosomes. One partner was Runx1, the murine homologue of the transcription factor AML-1, a target of human translocations in therapy-related leukemia. These findings indicate that etoposide triggers the formation of Mll gene fusions, a critical step for the development of treatment-induced leukemic transformation. | lld:pubmed |
| pubmed-article:14630694 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14630694 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14630694 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14630694 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14630694 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14630694 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14630694 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14630694 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14630694 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14630694 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14630694 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14630694 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14630694 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14630694 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14630694 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:14630694 | pubmed:issn | 1530-6860 | lld:pubmed |
| pubmed-article:14630694 | pubmed:author | pubmed-author:RellingMary... | lld:pubmed |
| pubmed-article:14630694 | pubmed:author | pubmed-author:BlancoJavier... | lld:pubmed |
| pubmed-article:14630694 | pubmed:author | pubmed-author:EdickMathew... | lld:pubmed |
| pubmed-article:14630694 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:14630694 | pubmed:volume | 18 | lld:pubmed |
| pubmed-article:14630694 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14630694 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14630694 | pubmed:pagination | 173-5 | lld:pubmed |
| pubmed-article:14630694 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:14630694 | pubmed:meshHeading | pubmed-meshheading:14630694... | lld:pubmed |
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| pubmed-article:14630694 | pubmed:meshHeading | pubmed-meshheading:14630694... | lld:pubmed |
| pubmed-article:14630694 | pubmed:meshHeading | pubmed-meshheading:14630694... | lld:pubmed |
| pubmed-article:14630694 | pubmed:meshHeading | pubmed-meshheading:14630694... | lld:pubmed |
| pubmed-article:14630694 | pubmed:meshHeading | pubmed-meshheading:14630694... | lld:pubmed |
| pubmed-article:14630694 | pubmed:meshHeading | pubmed-meshheading:14630694... | lld:pubmed |
| pubmed-article:14630694 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:14630694 | pubmed:articleTitle | Etoposide induces chimeric Mll gene fusions. | lld:pubmed |
| pubmed-article:14630694 | pubmed:affiliation | Department of Pharmaceutical Sciences, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA. | lld:pubmed |
| pubmed-article:14630694 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14630694 | lld:pubmed |
