| pubmed-article:14624642 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14624642 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:14624642 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:14624642 | lifeskim:mentions | umls-concept:C0450442 | lld:lifeskim |
| pubmed-article:14624642 | lifeskim:mentions | umls-concept:C0444454 | lld:lifeskim |
| pubmed-article:14624642 | lifeskim:mentions | umls-concept:C0065573 | lld:lifeskim |
| pubmed-article:14624642 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:14624642 | pubmed:dateCreated | 2003-11-19 | lld:pubmed |
| pubmed-article:14624642 | pubmed:abstractText | A new heterobifunctional linker containing an aldehyde-reactive aminooxy group and a thiol-reactive maleimide group, namely N-[4-(aminooxy)butyl]maleimide, was synthesized as a stable HCl salt by O-alkylation of either N-hydroxyphthalimide or N-(4-monomethoxytrityl)hydroxylamine, followed by N-alkylation of maleimide, in an overall yield of 18% (seven steps) or 29% (five steps), respectively. This heterobifunctional linker allowed a simple and efficient synthesis of a maleimide-containing thiol-reactive (18)F-labeling agent. Thus, N-[4-[(4-[(18)F]fluorobenzylidene)aminooxy]butyl]maleimide (specific activity: approximately 3000 Ci/mmol at end of synthesis) was synthesized in two steps involving the preparation of 4-[(18)F]fluorobenzaldehyde, followed by its aminooxy-aldehyde coupling reaction to the heterobifunctional linker, with an overall radiochemical yield of approximately 35% (decay corrected) within approximately 60 min from end of bombardment. Initial (18)F-labeling experiments were carried out using a thiol-containing tripeptide glutathione (GSH) and a 5'-thiol-functionalized oligodeoxynucleotide (5'-S-ODN) in phosphate-buffered saline (PBS, pH 7.5). After standing at room temperature for 10 min, the (18)F-labeled GSH and 5'-S-ODN were obtained in (18)F-labeling yields of approximately 70% and approximately 5% (decay-corrected), respectively. The heterobifunctional linker is easy to synthesize and provides a facile access to the maleimide-containing thiol-reactive (18)F-labeling agent, which could be advantageously employed in the development of (18)F-labeled biomomolecules for use with positron emission tomography. | lld:pubmed |
| pubmed-article:14624642 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14624642 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14624642 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14624642 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14624642 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14624642 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14624642 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14624642 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14624642 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14624642 | pubmed:issn | 1043-1802 | lld:pubmed |
| pubmed-article:14624642 | pubmed:author | pubmed-author:SatyamurthyNa... | lld:pubmed |
| pubmed-article:14624642 | pubmed:author | pubmed-author:BarrioJorge... | lld:pubmed |
| pubmed-article:14624642 | pubmed:author | pubmed-author:GambhirSanjiv... | lld:pubmed |
| pubmed-article:14624642 | pubmed:author | pubmed-author:PhelpsMichael... | lld:pubmed |
| pubmed-article:14624642 | pubmed:author | pubmed-author:ToyokuniTatsu... | lld:pubmed |
| pubmed-article:14624642 | pubmed:author | pubmed-author:WalshJoseph... | lld:pubmed |
| pubmed-article:14624642 | pubmed:author | pubmed-author:DominguezAlan... | lld:pubmed |
| pubmed-article:14624642 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14624642 | pubmed:volume | 14 | lld:pubmed |
| pubmed-article:14624642 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14624642 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14624642 | pubmed:pagination | 1253-9 | lld:pubmed |
| pubmed-article:14624642 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:14624642 | pubmed:meshHeading | pubmed-meshheading:14624642... | lld:pubmed |
| pubmed-article:14624642 | pubmed:meshHeading | pubmed-meshheading:14624642... | lld:pubmed |
| pubmed-article:14624642 | pubmed:meshHeading | pubmed-meshheading:14624642... | lld:pubmed |
| pubmed-article:14624642 | pubmed:meshHeading | pubmed-meshheading:14624642... | lld:pubmed |
| pubmed-article:14624642 | pubmed:meshHeading | pubmed-meshheading:14624642... | lld:pubmed |
| pubmed-article:14624642 | pubmed:meshHeading | pubmed-meshheading:14624642... | lld:pubmed |
| pubmed-article:14624642 | pubmed:articleTitle | Synthesis of a new heterobifunctional linker, N-[4-(aminooxy)butyl]maleimide, for facile access to a thiol-reactive 18F-labeling agent. | lld:pubmed |
| pubmed-article:14624642 | pubmed:affiliation | Crump Institute for Molecular Imaging, Department of Molecular & Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095-1770, USA. ttoyokuni@mednet.ucla.edu | lld:pubmed |
| pubmed-article:14624642 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14624642 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:14624642 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14624642 | lld:pubmed |
