| pubmed-article:14597987 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14597987 | lifeskim:mentions | umls-concept:C0007578 | lld:lifeskim |
| pubmed-article:14597987 | lifeskim:mentions | umls-concept:C0003209 | lld:lifeskim |
| pubmed-article:14597987 | lifeskim:mentions | umls-concept:C0021467 | lld:lifeskim |
| pubmed-article:14597987 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:14597987 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:14597987 | lifeskim:mentions | umls-concept:C1523116 | lld:lifeskim |
| pubmed-article:14597987 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
| pubmed-article:14597987 | lifeskim:mentions | umls-concept:C0127400 | lld:lifeskim |
| pubmed-article:14597987 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:14597987 | lifeskim:mentions | umls-concept:C0378018 | lld:lifeskim |
| pubmed-article:14597987 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:14597987 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:14597987 | pubmed:dateCreated | 2003-11-4 | lld:pubmed |
| pubmed-article:14597987 | pubmed:abstractText | The regulated expression of adhesion molecules on the surface of endothelial cells is a key process in the pathogenesis of inflammation. The saponin astragaloside IV (AS-IV), a 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosylcycloastragenol purified from the Chinese medical herb Astragalus membranaceus (Fisch) Bge. has been shown to have anti-inflammatory effects in vivo. In this study we have investigated the effect of AS-IV on cytokine-and LPS-stimulated expression of adhesion molecules in and leukocyte adhesion to endothelial cells. We have demonstrated that AS-IV significantly reduced the adhesion promoting activity of LPS-stimulated HUVECs for polymorph-nuclear leukocytes (PMNs) and the monocytic cell line THP-1. Furthermore, by using specific cell ELISAs we could show that AS-IV decreased the LPS-induced expression of E-selectin and VCAM-1 on the surface of HUVECs in a dose and time dependent manner, whereas the expression of ICAM-1 was not affected by AS-IV. AS-IV also inhibits TNFalpha-induced VCAM-1 expression. The saponin octyl-D-glucopyranoside had no effect on the LPS-induced expression of E-selectin and VCAM-1 excluding an unspecific detergent-like effect of AS-IV. Moreover, AS-IV significantly inhibited LPS- and TNFalpha-induced specific mRNA levels for E-selectin and VCAM-1. Finally, we could show that AS-IV completely abolished LPS- and TNFalpha-induced nuclear translocation of NF-kappaB and NF-kappaB DNA binding activity in endothelial cells. We conclude that the ability of AS-IV to inhibit the NF-kappaB pathway might be one under-lying mechanism contributing to its anti-inflammatory potential in vivo. | lld:pubmed |
| pubmed-article:14597987 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14597987 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14597987 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14597987 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14597987 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14597987 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14597987 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14597987 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14597987 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14597987 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14597987 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14597987 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14597987 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14597987 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14597987 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14597987 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14597987 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:14597987 | pubmed:issn | 0340-6245 | lld:pubmed |
| pubmed-article:14597987 | pubmed:author | pubmed-author:BinderBernd... | lld:pubmed |
| pubmed-article:14597987 | pubmed:author | pubmed-author:WojtaJohannJ | lld:pubmed |
| pubmed-article:14597987 | pubmed:author | pubmed-author:HufnaglPeterP | lld:pubmed |
| pubmed-article:14597987 | pubmed:author | pubmed-author:ZhangWei-Jian... | lld:pubmed |
| pubmed-article:14597987 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14597987 | pubmed:volume | 90 | lld:pubmed |
| pubmed-article:14597987 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14597987 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14597987 | pubmed:pagination | 904-14 | lld:pubmed |
| pubmed-article:14597987 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:14597987 | pubmed:meshHeading | pubmed-meshheading:14597987... | lld:pubmed |
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| pubmed-article:14597987 | pubmed:meshHeading | pubmed-meshheading:14597987... | lld:pubmed |
| pubmed-article:14597987 | pubmed:meshHeading | pubmed-meshheading:14597987... | lld:pubmed |
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| pubmed-article:14597987 | pubmed:meshHeading | pubmed-meshheading:14597987... | lld:pubmed |
| pubmed-article:14597987 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:14597987 | pubmed:articleTitle | Antiinflammatory activity of astragaloside IV is mediated by inhibition of NF-kappaB activation and adhesion molecule expression. | lld:pubmed |
| pubmed-article:14597987 | pubmed:affiliation | Department of Vascular Biology and Thrombosis Research, University of Vienna, Austria. | lld:pubmed |
| pubmed-article:14597987 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14597987 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
