| pubmed-article:14575548 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14575548 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:14575548 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:14575548 | lifeskim:mentions | umls-concept:C0005955 | lld:lifeskim |
| pubmed-article:14575548 | lifeskim:mentions | umls-concept:C0018133 | lld:lifeskim |
| pubmed-article:14575548 | lifeskim:mentions | umls-concept:C2348480 | lld:lifeskim |
| pubmed-article:14575548 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:14575548 | pubmed:dateCreated | 2003-10-24 | lld:pubmed |
| pubmed-article:14575548 | pubmed:abstractText | To explore the new approach to prevent graft versus host disease (GVHD) by purging ex vivo T lymphocytes of bone marrow graft through Fas-FasL way, FasL-cDNA was transfected into BALB/c mouse bon e marrow cells by liposome ex vivo. The transfected cells were cultured together with BAC (BALB/c x C57BL/6) mouse bone marrow graft. The mixing bone marrow graft was infused into BALB/c mouse recipients after 60Co-gamma irradiation. The mortality, manifestation and pathologic change of GVHD in recipient mice were observed. The CFU-S and Y chromosome from donor mice were detected. The results showed that compared with control group, the mortality in 60 days of the recipients in the experimental group decreased (20% vs 70%, P < 0.01) and the morbidity of GVHD lowered (40% vs 100%, P < 0.01). The CFU-S counts for all groups were at normal level on 20 days after transplantation. The Y chromosome from donor mice was discovered in 70% bone marrow nucleated cells of recipient mice survived over 2 months in the experimental group. It is concluded that mFasL-cDNA transfected mouse bone marrow cells prevent GVHD after culturing together with bone marrow graft, and accelerate hematopoietic reconstitution in recipient mice. | lld:pubmed |
| pubmed-article:14575548 | pubmed:language | chi | lld:pubmed |
| pubmed-article:14575548 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14575548 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14575548 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14575548 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14575548 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14575548 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14575548 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:14575548 | pubmed:issn | 1009-2137 | lld:pubmed |
| pubmed-article:14575548 | pubmed:author | pubmed-author:ZouPingP | lld:pubmed |
| pubmed-article:14575548 | pubmed:author | pubmed-author:MaYan-PingYP | lld:pubmed |
| pubmed-article:14575548 | pubmed:author | pubmed-author:LiuLing-BoLB | lld:pubmed |
| pubmed-article:14575548 | pubmed:author | pubmed-author:LiAi-XiangAX | lld:pubmed |
| pubmed-article:14575548 | pubmed:author | pubmed-author:XuZhi-LiangZL | lld:pubmed |
| pubmed-article:14575548 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14575548 | pubmed:volume | 11 | lld:pubmed |
| pubmed-article:14575548 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14575548 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14575548 | pubmed:pagination | 512-5 | lld:pubmed |
| pubmed-article:14575548 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:14575548 | pubmed:meshHeading | pubmed-meshheading:14575548... | lld:pubmed |
| pubmed-article:14575548 | pubmed:meshHeading | pubmed-meshheading:14575548... | lld:pubmed |
| pubmed-article:14575548 | pubmed:meshHeading | pubmed-meshheading:14575548... | lld:pubmed |
| pubmed-article:14575548 | pubmed:meshHeading | pubmed-meshheading:14575548... | lld:pubmed |
| pubmed-article:14575548 | pubmed:meshHeading | pubmed-meshheading:14575548... | lld:pubmed |
| pubmed-article:14575548 | pubmed:meshHeading | pubmed-meshheading:14575548... | lld:pubmed |
| pubmed-article:14575548 | pubmed:meshHeading | pubmed-meshheading:14575548... | lld:pubmed |
| pubmed-article:14575548 | pubmed:meshHeading | pubmed-meshheading:14575548... | lld:pubmed |
| pubmed-article:14575548 | pubmed:meshHeading | pubmed-meshheading:14575548... | lld:pubmed |
| pubmed-article:14575548 | pubmed:meshHeading | pubmed-meshheading:14575548... | lld:pubmed |
| pubmed-article:14575548 | pubmed:meshHeading | pubmed-meshheading:14575548... | lld:pubmed |
| pubmed-article:14575548 | pubmed:meshHeading | pubmed-meshheading:14575548... | lld:pubmed |
| pubmed-article:14575548 | pubmed:meshHeading | pubmed-meshheading:14575548... | lld:pubmed |
| pubmed-article:14575548 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:14575548 | pubmed:articleTitle | [FasL-cDNA transfected into mouse bone marrow cells ex vivo to prevent graft versus host disease]. | lld:pubmed |
| pubmed-article:14575548 | pubmed:affiliation | Department of Pediatrics, People's Hospital, Wuhan University, Wuhan 430060, China. | lld:pubmed |
| pubmed-article:14575548 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14575548 | pubmed:publicationType | English Abstract | lld:pubmed |
| pubmed-article:14575548 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
