| pubmed-article:14565328 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14565328 | lifeskim:mentions | umls-concept:C0026882 | lld:lifeskim |
| pubmed-article:14565328 | lifeskim:mentions | umls-concept:C0017431 | lld:lifeskim |
| pubmed-article:14565328 | lifeskim:mentions | umls-concept:C0681828 | lld:lifeskim |
| pubmed-article:14565328 | lifeskim:mentions | umls-concept:C1579410 | lld:lifeskim |
| pubmed-article:14565328 | pubmed:issue | 5-8 | lld:pubmed |
| pubmed-article:14565328 | pubmed:dateCreated | 2003-10-20 | lld:pubmed |
| pubmed-article:14565328 | pubmed:abstractText | In spite of a rather long period of investigations, the problem of HIV drug resistance remains unsolved, and more that, at present HIV-1 mutants resistant to all known nucleoside inhibitors being used in clinical therapy against the human immunodeficiency syndrome are discovered. In this study we selected HIV-1 mutants resistant to the nucleoside inhibitors of HIV reverse transcriptase (NRTI): 3'-azido-2',3'-dideoxythymidine (AZT), 5'-phosphit 3'-azido-2',3'-dideoxythymidine (ph-AZT), dideoxyinosine (ddI) and didehydrodeoxythymidine (d4T). Selection of resistant mutants was carried out by gradually increasing of drug concentration in the culture medium during propagation of the HIV-1EVK on fresh MT-4 cells. Phenotypic resistance was defined as an increase in ID50 of 160-fold for AZT, 8 for ph-AZT, 10 for ddI, 7 for d4T. In comparison studies it was determined that the viral resistance to these drugs was appeared variously in a similar conditions and duration of selection. The nucleotide sequences of the RT region of the HIV-1 variants were compared with the HIV-1EVK from "0" passage. For some of selected HIV-1 mutants NRTI resistance mutations were detected. Selected AZT resistant variants contained amino acid substitutions in positions D67A and K70R. Our studies was not revealed substitution at position 75 for ph-AZT resistant variants, whereas substitution at position L214F have been observed in both experiments using AZT and ph-AZT. Selected d4T resistant mutants contained amino acid substitutions in positions N54D and P52R. Selected ddI resistant mutants contained only one amino acid substitution in position P143S. Collection of drug-resistant mutants should prove to be a convenient tool for rapid investigations a new antiretroviral agents on cross drug-resistance. | lld:pubmed |
| pubmed-article:14565328 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14565328 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14565328 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14565328 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14565328 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14565328 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14565328 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14565328 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14565328 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14565328 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14565328 | pubmed:issn | 1525-7770 | lld:pubmed |
| pubmed-article:14565328 | pubmed:author | pubmed-author:PokrovskyAA | lld:pubmed |
| pubmed-article:14565328 | pubmed:author | pubmed-author:PlyasunovaOO | lld:pubmed |
| pubmed-article:14565328 | pubmed:author | pubmed-author:GashnikovaNN | lld:pubmed |
| pubmed-article:14565328 | pubmed:author | pubmed-author:KiselevaYY | lld:pubmed |
| pubmed-article:14565328 | pubmed:author | pubmed-author:FedyukNN | lld:pubmed |
| pubmed-article:14565328 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14565328 | pubmed:volume | 22 | lld:pubmed |
| pubmed-article:14565328 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14565328 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14565328 | pubmed:pagination | 991-4 | lld:pubmed |
| pubmed-article:14565328 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:14565328 | pubmed:meshHeading | pubmed-meshheading:14565328... | lld:pubmed |
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| pubmed-article:14565328 | pubmed:meshHeading | pubmed-meshheading:14565328... | lld:pubmed |
| pubmed-article:14565328 | pubmed:articleTitle | In vitro study of resistance-associated genotypic mutations to nucleoside analogs. | lld:pubmed |
| pubmed-article:14565328 | pubmed:affiliation | State Research Center of Virology and Biotechnology Vector, Institute of Molecular Biology, Koltsovo, Novosibirsk Region, Russia. gapv@online.sinor.ru | lld:pubmed |
| pubmed-article:14565328 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14565328 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
