| pubmed-article:14563210 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14563210 | lifeskim:mentions | umls-concept:C0024660 | lld:lifeskim |
| pubmed-article:14563210 | lifeskim:mentions | umls-concept:C0597484 | lld:lifeskim |
| pubmed-article:14563210 | lifeskim:mentions | umls-concept:C0521116 | lld:lifeskim |
| pubmed-article:14563210 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:14563210 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:14563210 | pubmed:issue | Pt 2 | lld:pubmed |
| pubmed-article:14563210 | pubmed:dateCreated | 2004-1-5 | lld:pubmed |
| pubmed-article:14563210 | pubmed:abstractText | Stimulation of mammalian cells often results in an increase in the intracellular Na(+) concentration, brought about by Na(+) influx into the cell via Na(+)-permeable ion channels. In some cell types, particularly renal epithelia and mast cells, non-hydrolysable analogues of GTP, such as GTP[S] (guanosine 5'-[gamma-thio]triphosphate), activate a non-voltage-activated Na(+)-selective current. We have carried out whole-cell patch-clamp experiments to examine how GTP[S] activates the Na(+) current in a rat mast cell line. The ability of GTP[S] to activate Na(+) influx was prevented by including GTP in the pipette solution, indicating the involvement of small G-proteins. Brefeldin A and Arf-1-(2-17), inhibitors of Arf-1 (ADP-ribosylation factor-1) proteins, suppressed the activation of Na(+) entry by GTP[S]. However, non-active succinylated Arf-1-(2-17) or an N-terminal myristoylated peptide directed towards Arf-5 were ineffective. Arf proteins modulate the cytoskeleton, and disruption of the cytoskeleton with cytochalasin D or its stabilization with phalloidin impaired the development of the Na(+) current. Disaggregation of microtubules was without effect. Dialysis with cAMP or inhibition of cAMP phosphodiesterase with caffeine both decreased the extent of Na(+) entry, and this was not prevented by pre-treatment with broad-spectrum protein kinase inhibitors. Collectively, our results suggest that the mechanism of activation of a mammalian non-voltage-activated Na(+)-selective current requires an Arf small G-protein, most probably Arf-1. | lld:pubmed |
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| pubmed-article:14563210 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14563210 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14563210 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14563210 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14563210 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:14563210 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14563210 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14563210 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:14563210 | pubmed:issn | 1470-8728 | lld:pubmed |
| pubmed-article:14563210 | pubmed:author | pubmed-author:ParekhAnant... | lld:pubmed |
| pubmed-article:14563210 | pubmed:author | pubmed-author:StraubeSebast... | lld:pubmed |
| pubmed-article:14563210 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:14563210 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:14563210 | pubmed:volume | 377 | lld:pubmed |
| pubmed-article:14563210 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14563210 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14563210 | pubmed:pagination | 539-44 | lld:pubmed |
| pubmed-article:14563210 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:14563210 | pubmed:meshHeading | pubmed-meshheading:14563210... | lld:pubmed |
| pubmed-article:14563210 | pubmed:meshHeading | pubmed-meshheading:14563210... | lld:pubmed |
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| pubmed-article:14563210 | pubmed:meshHeading | pubmed-meshheading:14563210... | lld:pubmed |
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| pubmed-article:14563210 | pubmed:meshHeading | pubmed-meshheading:14563210... | lld:pubmed |
| pubmed-article:14563210 | pubmed:meshHeading | pubmed-meshheading:14563210... | lld:pubmed |
| pubmed-article:14563210 | pubmed:meshHeading | pubmed-meshheading:14563210... | lld:pubmed |
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| pubmed-article:14563210 | pubmed:meshHeading | pubmed-meshheading:14563210... | lld:pubmed |
| pubmed-article:14563210 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:14563210 | pubmed:articleTitle | Arf-1 (ADP-ribosylation factor-1) is involved in the activation of a mammalian Na+-selective current. | lld:pubmed |
| pubmed-article:14563210 | pubmed:affiliation | Laboratory of Cellular and Molecular Signalling, Department of Physiology, University of Oxford, Parks Road, Oxford OX1 3PT, UK. | lld:pubmed |
| pubmed-article:14563210 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14563210 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:64310 | entrezgene:pubmed | pubmed-article:14563210 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:14563210 | lld:entrezgene |
