| pubmed-article:1443155 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1443155 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:1443155 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:1443155 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:1443155 | lifeskim:mentions | umls-concept:C1179002 | lld:lifeskim |
| pubmed-article:1443155 | lifeskim:mentions | umls-concept:C1704259 | lld:lifeskim |
| pubmed-article:1443155 | lifeskim:mentions | umls-concept:C1705987 | lld:lifeskim |
| pubmed-article:1443155 | pubmed:issue | 5 Pt 1 | lld:pubmed |
| pubmed-article:1443155 | pubmed:dateCreated | 1992-12-23 | lld:pubmed |
| pubmed-article:1443155 | pubmed:abstractText | The utility of a transgenic murine model of cystic fibrosis (CF) lung disease will likely depend on whether the mouse's proximal airway epithelium is characterized by Na(+)- and Cl(-)-conductive pathways comparable to those found in human airways. Therefore, the electrophysiological properties of primary cultures of mouse tracheal epithelium (MTE) were investigated using double-barreled, Cl(-)-selective microelectrodes. Epithelial cells isolated from freshly excised mouse tracheae formed confluent polarized monolayers on permeable collagen supports and developed significant transepithelial potential differences (approximately -10 mV) within 5-6 days postseeding. Under basal conditions, the MTE monolayers had an equivalent short-circuit current (Ieq) of -21.1 +/- 2.1 microA/cm2 and a transepithelial resistance of 424 +/- 49 omega.cm2. Intracellular measurements indicated that the apical (Va) and basolateral (Vb) membrane potential differences were -16.9 +/- 1.5 and -25.4 +/- 1.5 mV, respectively; apical membrane fractional resistance was 0.36 +/- 0.03; and intracellular Cl- activity was 56.1 +/- 2.3 mM. The presence of an apical Na+ conductance was demonstrated by luminal amiloride application (10(-4)M), which decreased Ieq, hyperpolarized Va, and increased the fractional resistance of the apical membrane. The presence of an apical Cl- conductance was demonstrated by substitution of Cl- with gluconate in the luminal bath, which decreased intracellular Cl- activity and increased the fractional resistance of the apical membrane. Luminal application of ATP (10(-4) M was also found to increase the rate of Cl- secretion.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:1443155 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1443155 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1443155 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1443155 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1443155 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1443155 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1443155 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1443155 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1443155 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1443155 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1443155 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1443155 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:1443155 | pubmed:issn | 0002-9513 | lld:pubmed |
| pubmed-article:1443155 | pubmed:author | pubmed-author:BoucherR CRC | lld:pubmed |
| pubmed-article:1443155 | pubmed:author | pubmed-author:ClarkeL LLL | lld:pubmed |
| pubmed-article:1443155 | pubmed:author | pubmed-author:BurnsK AKA | lld:pubmed |
| pubmed-article:1443155 | pubmed:author | pubmed-author:Van ScottM... | lld:pubmed |
| pubmed-article:1443155 | pubmed:author | pubmed-author:BayleJ YJY | lld:pubmed |
| pubmed-article:1443155 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1443155 | pubmed:volume | 263 | lld:pubmed |
| pubmed-article:1443155 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1443155 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1443155 | pubmed:pagination | L519-25 | lld:pubmed |
| pubmed-article:1443155 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:1443155 | pubmed:meshHeading | pubmed-meshheading:1443155-... | lld:pubmed |
| pubmed-article:1443155 | pubmed:meshHeading | pubmed-meshheading:1443155-... | lld:pubmed |
| pubmed-article:1443155 | pubmed:meshHeading | pubmed-meshheading:1443155-... | lld:pubmed |
| pubmed-article:1443155 | pubmed:meshHeading | pubmed-meshheading:1443155-... | lld:pubmed |
| pubmed-article:1443155 | pubmed:meshHeading | pubmed-meshheading:1443155-... | lld:pubmed |
| pubmed-article:1443155 | pubmed:meshHeading | pubmed-meshheading:1443155-... | lld:pubmed |
| pubmed-article:1443155 | pubmed:meshHeading | pubmed-meshheading:1443155-... | lld:pubmed |
| pubmed-article:1443155 | pubmed:meshHeading | pubmed-meshheading:1443155-... | lld:pubmed |
| pubmed-article:1443155 | pubmed:meshHeading | pubmed-meshheading:1443155-... | lld:pubmed |
| pubmed-article:1443155 | pubmed:meshHeading | pubmed-meshheading:1443155-... | lld:pubmed |
| pubmed-article:1443155 | pubmed:meshHeading | pubmed-meshheading:1443155-... | lld:pubmed |
| pubmed-article:1443155 | pubmed:meshHeading | pubmed-meshheading:1443155-... | lld:pubmed |
| pubmed-article:1443155 | pubmed:meshHeading | pubmed-meshheading:1443155-... | lld:pubmed |
| pubmed-article:1443155 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1443155 | pubmed:articleTitle | Sodium- and chloride-conductive pathways in cultured mouse tracheal epithelium. | lld:pubmed |
| pubmed-article:1443155 | pubmed:affiliation | Department of Medicine, University of North Carolina, Chapel Hill 27599-7020. | lld:pubmed |
| pubmed-article:1443155 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1443155 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1443155 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1443155 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1443155 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1443155 | lld:pubmed |
