| pubmed-article:1420436 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1420436 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:1420436 | lifeskim:mentions | umls-concept:C0028953 | lld:lifeskim |
| pubmed-article:1420436 | lifeskim:mentions | umls-concept:C0066424 | lld:lifeskim |
| pubmed-article:1420436 | lifeskim:mentions | umls-concept:C1521827 | lld:lifeskim |
| pubmed-article:1420436 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:1420436 | pubmed:dateCreated | 1992-12-22 | lld:pubmed |
| pubmed-article:1420436 | pubmed:abstractText | A method is reported for conjugating an analog of 4'-(aminomethyl)-4,5',8- trimethylpsoralen to methylphophonate oligonucleotides. This method enables the psoralen moiety to be coupled to the phosphonate backbone between any two desired bases in a sequence. When hybridized to a target mRNA, the psoralen moiety can be directed toward a uridine base and, in turn, can undergo a photo-addition reaction with the target under UV irradiation at 365 nm. Several different non-nucleotide-based amino-linker reagents have been prepared for incorporation into methylphosphonate oligonucleotides by standard phosphonamidite chemistry. In addition, an N-hydroxysuccinimide activated ester analog of 4'-[(3-carboxypropionamido)methyl]-4,5',8- trimethylpsoralen has been synthesized for conjugation to the amino-linker moieties. Using this approach, we have prepared a number of psoralen-methylphosphonate-oligonucleotide conjugates which are complementary to the chimeric bcr/abl mRNA associated with chronic myelogenous leukemia. Solution hybridization studies with a 440-base subfragment of the bcr/abl RNA have shown that the psoralen moiety does not adversely affect duplex stability. Polyacrylamide gel electrophoresis analyses have demonstrated that the psoralen-oligonucleotide conjugates undergo photo-addition to the RNA in a sequence-specific manner. Optimal photo-addition occurs when the psoralen moiety is inserted adjacent to one or more adenine residues in the oligonucleotide sequence, particularly between adenine and thymine (5'-3'). This internal labeling approach greatly increases the number of potential target sites available for photo-cross-linking experiments. | lld:pubmed |
| pubmed-article:1420436 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1420436 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1420436 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1420436 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1420436 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1420436 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1420436 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1420436 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1420436 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1420436 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1420436 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1420436 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1420436 | pubmed:issn | 1043-1802 | lld:pubmed |
| pubmed-article:1420436 | pubmed:author | pubmed-author:ArnoldL JLJJr | lld:pubmed |
| pubmed-article:1420436 | pubmed:author | pubmed-author:ReynoldsM AMA | lld:pubmed |
| pubmed-article:1420436 | pubmed:author | pubmed-author:BeckT ATA | lld:pubmed |
| pubmed-article:1420436 | pubmed:author | pubmed-author:VaghefiM MMM | lld:pubmed |
| pubmed-article:1420436 | pubmed:author | pubmed-author:HogrefeR IRI | lld:pubmed |
| pubmed-article:1420436 | pubmed:author | pubmed-author:McCaffreyAA | lld:pubmed |
| pubmed-article:1420436 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1420436 | pubmed:volume | 3 | lld:pubmed |
| pubmed-article:1420436 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1420436 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1420436 | pubmed:pagination | 366-74 | lld:pubmed |
| pubmed-article:1420436 | pubmed:dateRevised | 2001-3-23 | lld:pubmed |
| pubmed-article:1420436 | pubmed:meshHeading | pubmed-meshheading:1420436-... | lld:pubmed |
| pubmed-article:1420436 | pubmed:meshHeading | pubmed-meshheading:1420436-... | lld:pubmed |
| pubmed-article:1420436 | pubmed:meshHeading | pubmed-meshheading:1420436-... | lld:pubmed |
| pubmed-article:1420436 | pubmed:meshHeading | pubmed-meshheading:1420436-... | lld:pubmed |
| pubmed-article:1420436 | pubmed:meshHeading | pubmed-meshheading:1420436-... | lld:pubmed |
| pubmed-article:1420436 | pubmed:meshHeading | pubmed-meshheading:1420436-... | lld:pubmed |
| pubmed-article:1420436 | pubmed:meshHeading | pubmed-meshheading:1420436-... | lld:pubmed |
| pubmed-article:1420436 | pubmed:meshHeading | pubmed-meshheading:1420436-... | lld:pubmed |
| pubmed-article:1420436 | pubmed:meshHeading | pubmed-meshheading:1420436-... | lld:pubmed |
| pubmed-article:1420436 | pubmed:meshHeading | pubmed-meshheading:1420436-... | lld:pubmed |
| pubmed-article:1420436 | pubmed:meshHeading | pubmed-meshheading:1420436-... | lld:pubmed |
| pubmed-article:1420436 | pubmed:meshHeading | pubmed-meshheading:1420436-... | lld:pubmed |
| pubmed-article:1420436 | pubmed:articleTitle | A non-nucleotide-based linking method for the preparation of psoralen-derivatized methylphosphonate oligonucleotides. | lld:pubmed |
| pubmed-article:1420436 | pubmed:affiliation | Genta Inc., San Diego, California 92121. | lld:pubmed |
| pubmed-article:1420436 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1420436 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1420436 | lld:pubmed |
