| pubmed-article:1382847 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1382847 | lifeskim:mentions | umls-concept:C0030705 | lld:lifeskim |
| pubmed-article:1382847 | lifeskim:mentions | umls-concept:C0330390 | lld:lifeskim |
| pubmed-article:1382847 | lifeskim:mentions | umls-concept:C0005767 | lld:lifeskim |
| pubmed-article:1382847 | lifeskim:mentions | umls-concept:C2239176 | lld:lifeskim |
| pubmed-article:1382847 | lifeskim:mentions | umls-concept:C0079722 | lld:lifeskim |
| pubmed-article:1382847 | lifeskim:mentions | umls-concept:C0034790 | lld:lifeskim |
| pubmed-article:1382847 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:1382847 | lifeskim:mentions | umls-concept:C0457083 | lld:lifeskim |
| pubmed-article:1382847 | pubmed:issue | 21 | lld:pubmed |
| pubmed-article:1382847 | pubmed:dateCreated | 1992-11-18 | lld:pubmed |
| pubmed-article:1382847 | pubmed:abstractText | To determine a possibly restricted T-cell receptor (TCR) repertoire in tumor-infiltrating lymphocytes (TIL) in response to tumor-associated antigens in patients with hepatocellular carcinoma (HCC), freshly isolated TIL (n = 5) and peripheral blood lymphocytes (PBL; n = 6; 3 paired with TIL) were studied for expression of TCR variable (V) beta regions. RNA purified from TIL or PBL was reverse-transcribed into complementary DNA. This complementary DNA was amplified by quantitative polymerase chain reaction with 22 primers specific for 20 TCR V beta gene families and a 3' constant (C) beta primer. As a reference for later quantitation, a fragment of TCR C alpha was coamplified with each V beta region. Using 32P-labeled 3' primers, the percentage of total V beta expression was calculated by measuring the cpm of each of the amplified products. In contrast to PBL of 6 control, healthy individuals, whose range of expression of each TCR V beta gene varied from 0 to 13%, the expression of some V beta genes in HCC TIL was as high as 33%, indicating a restricted TCR V beta usage in HCC TIL. When polymerase chain reaction-amplified complementary DNAs of the V beta 1 or V beta 3 genes obtained from two TIL preparations were cloned and sequenced, the same rearrangements were found in the majority of DNA clones. The particular V beta genes that were over- or underrepresented in TIL varied among the patients. In 3 of 6 PBL and 3 of 5 TIL, the V beta 3 gene was expressed with a relatively high frequency. The V beta 4 gene expression was consistently low in patients' TIL or PBL. In 3 paired PBL and TIL, V beta expression was similar. In 5 of 6 cases, HCC PBL had different TCR V beta frequencies from those seen in normal PBL. This analysis of TCR V beta usage in freshly isolated TIL and in PBL indicated that T-lymphocytes in patients with HCC might have restricted immunological reactivity and that V beta 3-restricted TIL might represent antitumor effector cells. | lld:pubmed |
| pubmed-article:1382847 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1382847 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1382847 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1382847 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1382847 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1382847 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1382847 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1382847 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:1382847 | pubmed:issn | 0008-5472 | lld:pubmed |
| pubmed-article:1382847 | pubmed:author | pubmed-author:HerbermanR... | lld:pubmed |
| pubmed-article:1382847 | pubmed:author | pubmed-author:TruccoMM | lld:pubmed |
| pubmed-article:1382847 | pubmed:author | pubmed-author:WhitesideT... | lld:pubmed |
| pubmed-article:1382847 | pubmed:author | pubmed-author:WeidmannEE | lld:pubmed |
| pubmed-article:1382847 | pubmed:author | pubmed-author:GiordaRR | lld:pubmed |
| pubmed-article:1382847 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1382847 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:1382847 | pubmed:volume | 52 | lld:pubmed |
| pubmed-article:1382847 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1382847 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1382847 | pubmed:pagination | 5913-20 | lld:pubmed |
| pubmed-article:1382847 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:1382847 | pubmed:meshHeading | pubmed-meshheading:1382847-... | lld:pubmed |
| pubmed-article:1382847 | pubmed:meshHeading | pubmed-meshheading:1382847-... | lld:pubmed |
| pubmed-article:1382847 | pubmed:meshHeading | pubmed-meshheading:1382847-... | lld:pubmed |
| pubmed-article:1382847 | pubmed:meshHeading | pubmed-meshheading:1382847-... | lld:pubmed |
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| pubmed-article:1382847 | pubmed:meshHeading | pubmed-meshheading:1382847-... | lld:pubmed |
| pubmed-article:1382847 | pubmed:meshHeading | pubmed-meshheading:1382847-... | lld:pubmed |
| pubmed-article:1382847 | pubmed:meshHeading | pubmed-meshheading:1382847-... | lld:pubmed |
| pubmed-article:1382847 | pubmed:meshHeading | pubmed-meshheading:1382847-... | lld:pubmed |
| pubmed-article:1382847 | pubmed:meshHeading | pubmed-meshheading:1382847-... | lld:pubmed |
| pubmed-article:1382847 | pubmed:meshHeading | pubmed-meshheading:1382847-... | lld:pubmed |
| pubmed-article:1382847 | pubmed:meshHeading | pubmed-meshheading:1382847-... | lld:pubmed |
| pubmed-article:1382847 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1382847 | pubmed:articleTitle | The T-cell receptor V beta gene usage in tumor-infiltrating lymphocytes and blood of patients with hepatocellular carcinoma. | lld:pubmed |
| pubmed-article:1382847 | pubmed:affiliation | Department of Pathology, University of Pittsburgh School of Medicine, Pennsylvania. | lld:pubmed |
| pubmed-article:1382847 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1382847 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1382847 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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