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| pubmed-article:1382605 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:1382605 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:1382605 | pubmed:dateCreated | 1992-11-2 | lld:pubmed |
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| pubmed-article:1382605 | pubmed:abstractText | Two human homologues of protein kinase C-epsilon (E1 and E2) were isolated from two distinct cDNA libraries. Sequence comparisons to PKC-epsilon cDNAs from several species indicated that each of these human epsilon clones contained cloning artifacts. Thus, a composite PKC-epsilon (E3) clone was derived from clones E1 and E2. Human PKC-epsilon (E3) has an overall sequence identity of 90-92% at the nucleotide level compared to the previously characterized mouse, rat and rabbit clones. At the amino acid level, the deduced human epsilon sequence shows a 98-99% identity with the mouse, rat and rabbit sequences. Expression of the human PKC-epsilon clone in Sf9 cells confirmed that the recombinant protein displayed protein kinase C activity and phorbol ester binding activity. The recombinant protein was also recognized by two distinct epsilon-specific polyclonal antibodies. | lld:pubmed |
| pubmed-article:1382605 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:1382605 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1382605 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1382605 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:1382605 | pubmed:issn | 0006-3002 | lld:pubmed |
| pubmed-article:1382605 | pubmed:author | pubmed-author:BallasL MLM | lld:pubmed |
| pubmed-article:1382605 | pubmed:author | pubmed-author:HolmesWW | lld:pubmed |
| pubmed-article:1382605 | pubmed:author | pubmed-author:BurnsD JDJ | lld:pubmed |
| pubmed-article:1382605 | pubmed:author | pubmed-author:LoomisC RCR | lld:pubmed |
| pubmed-article:1382605 | pubmed:author | pubmed-author:BastyHH | lld:pubmed |
| pubmed-article:1382605 | pubmed:author | pubmed-author:StricklandM... | lld:pubmed |
| pubmed-article:1382605 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1382605 | pubmed:day | 24 | lld:pubmed |
| pubmed-article:1382605 | pubmed:volume | 1132 | lld:pubmed |
| pubmed-article:1382605 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1382605 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1382605 | pubmed:pagination | 154-60 | lld:pubmed |
| pubmed-article:1382605 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:1382605 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1382605 | pubmed:articleTitle | Sequence and expression of human protein kinase C-epsilon. | lld:pubmed |
| pubmed-article:1382605 | pubmed:affiliation | Sphinx Pharmaceuticals Corporation, Molecular Biology Section, Durham, NC 27717. | lld:pubmed |
| pubmed-article:1382605 | pubmed:publicationType | Journal Article | lld:pubmed |
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