| pubmed-article:1378422 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1378422 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:1378422 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:1378422 | lifeskim:mentions | umls-concept:C0023516 | lld:lifeskim |
| pubmed-article:1378422 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
| pubmed-article:1378422 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:1378422 | lifeskim:mentions | umls-concept:C0003316 | lld:lifeskim |
| pubmed-article:1378422 | lifeskim:mentions | umls-concept:C0023546 | lld:lifeskim |
| pubmed-article:1378422 | lifeskim:mentions | umls-concept:C0205275 | lld:lifeskim |
| pubmed-article:1378422 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:1378422 | pubmed:dateCreated | 1992-8-19 | lld:pubmed |
| pubmed-article:1378422 | pubmed:abstractText | Human blood polymorphonuclear (PMN) leucocytes and human leucocytes of the HL-60 line, which were induced to differentiate by 1,25-dihydroxyvitamin D3, express stereospecific receptors for the potent chemotactic mediator, leukotriene B4 (LTB4), that is derived by 5-lipoxygenation from arachidonic acid. Monoclonal antibodies to LTB4 receptors (LTB4-R) were generated by immunizing BALB/c mice with partially purified PMN leucocyte membrane proteins, and fusing their splenocytes with P3X63Ag8 mouse myeloma cells. Hybridoma supernatants were screened initially by binding to PMN leucocyte LTB4-R protein, which had been affinity cross-linked with aminopropylamide (APA)-LTB4 and immobilized in plastic wells through attachment of the linked APA-LTB4 to adherent Fab of monoclonal anti-LTB4. Of the three clones producing antibodies which bound to LTB4-R, 0.5 mg/ml of one IgG3k antibody, termed E2, precipitated over 90% of the [3H]LTB4-binding activity of solubilized PMN leucocyte membrane proteins. E2 also bound to a radiolabelled protein of 70,000-80,000 MW from 125I-labelled PMN leucocyte membranes [35S]-labelled HL-60 cell membranes, and PMN leucocyte membranes affinity-labelled with [3H]APA-LTB4, that was identical in size to the LTB4-R precipitated by the rabbit IgG anti-idiotypic antibodies. E2 did not bind to intact PMN leucocytes or modify the binding of [3H]LTB4 by PMN leucocytes. The binding of E2 to LTB4-R in purified membranes of PMN leucocytes was less than one-fourth of that observed for the anti-idiotypic antibodies, but increased substantially after solubilization of the LTB4-R. The E2 monoclonal antibody thus recognizes a partially latent substituent of LTB4-R, which does not contribute to combining site function. | lld:pubmed |
| pubmed-article:1378422 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1378422 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1378422 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1378422 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1378422 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1378422 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1378422 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1378422 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1378422 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1378422 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1378422 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1378422 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1378422 | pubmed:month | May | lld:pubmed |
| pubmed-article:1378422 | pubmed:issn | 0019-2805 | lld:pubmed |
| pubmed-article:1378422 | pubmed:author | pubmed-author:GoetzlE JEJ | lld:pubmed |
| pubmed-article:1378422 | pubmed:author | pubmed-author:BoggsJ MJM | lld:pubmed |
| pubmed-article:1378422 | pubmed:author | pubmed-author:YoungR NRN | lld:pubmed |
| pubmed-article:1378422 | pubmed:author | pubmed-author:HarveyJ PJP | lld:pubmed |
| pubmed-article:1378422 | pubmed:author | pubmed-author:KooC HCH | lld:pubmed |
| pubmed-article:1378422 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1378422 | pubmed:volume | 76 | lld:pubmed |
| pubmed-article:1378422 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1378422 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1378422 | pubmed:pagination | 122-8 | lld:pubmed |
| pubmed-article:1378422 | pubmed:dateRevised | 2010-9-7 | lld:pubmed |
| pubmed-article:1378422 | pubmed:meshHeading | pubmed-meshheading:1378422-... | lld:pubmed |
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| pubmed-article:1378422 | pubmed:meshHeading | pubmed-meshheading:1378422-... | lld:pubmed |
| pubmed-article:1378422 | pubmed:meshHeading | pubmed-meshheading:1378422-... | lld:pubmed |
| pubmed-article:1378422 | pubmed:meshHeading | pubmed-meshheading:1378422-... | lld:pubmed |
| pubmed-article:1378422 | pubmed:meshHeading | pubmed-meshheading:1378422-... | lld:pubmed |
| pubmed-article:1378422 | pubmed:meshHeading | pubmed-meshheading:1378422-... | lld:pubmed |
| pubmed-article:1378422 | pubmed:meshHeading | pubmed-meshheading:1378422-... | lld:pubmed |
| pubmed-article:1378422 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1378422 | pubmed:articleTitle | Mouse monoclonal antibody to a latent epitope of leucocyte receptors for leukotriene B4. | lld:pubmed |
| pubmed-article:1378422 | pubmed:affiliation | Department of Medicine, University of California Medical Center, San Francisco 94143-0724. | lld:pubmed |
| pubmed-article:1378422 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1378422 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
