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pubmed-article:1377511lifeskim:mentionsumls-concept:C0023607lld:lifeskim
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pubmed-article:1377511pubmed:issue3lld:pubmed
pubmed-article:1377511pubmed:dateCreated1992-7-31lld:pubmed
pubmed-article:1377511pubmed:abstractTextOur primary objective for this study was to determine whether steady-state amounts of alpha- and LH beta-subunit mRNAs in the anterior pituitary are altered during sexual maturation in the bovine female. A secondary objective was to determine whether 17 beta-estradiol (E2) alters amounts of LH subunit mRNAs before onset of puberty. Heifers (7 mo old) were assigned to one of three treatments: 1) ovariectomized (OVX, n = 16); 2) OVX and administered E2 (OVXE, n = 16); or 3) ovary-intact (INTACT, n = 20). Pituitaries were collected at an estimated 120 days before onset of puberty (prepuberty) or 25 days before onset of puberty (peripuberty). Six INTACT heifers were used to determine time of puberty during the experimental period, and their pituitaries were collected 40 h after administration of prostaglandin F2 alpha (postpubertal INTACT group). Relative amounts of mRNAs for LH subunits in each pituitary were determined by Northern analysis and scanning densitometry. Amounts of alpha- and LH beta-subunit mRNAs were lower in pituitaries of INTACT heifers and OVXE heifers, regardless of stage of sexual maturation, than in those of OVX heifers. Amounts of alpha-subunit mRNA were similar in OVXE and INTACT heifers regardless of stage of sexual maturation. Amounts of LH beta-subunit mRNA did not change during sexual maturation in heifers in the INTACT group. Concentrations of E2 were higher and LH beta-subunit mRNA were lower in heifers from the prepubertal OVXE group than in heifers in all other treatment groups.(ABSTRACT TRUNCATED AT 250 WORDS)lld:pubmed
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pubmed-article:1377511pubmed:authorpubmed-author:KojimaNNlld:pubmed
pubmed-article:1377511pubmed:authorpubmed-author:GrotjanH EHElld:pubmed
pubmed-article:1377511pubmed:authorpubmed-author:KinderJ EJElld:pubmed
pubmed-article:1377511pubmed:authorpubmed-author:KittokR JRJlld:pubmed
pubmed-article:1377511pubmed:authorpubmed-author:HamernikD LDLlld:pubmed
pubmed-article:1377511pubmed:authorpubmed-author:WolfeM WMWlld:pubmed
pubmed-article:1377511pubmed:authorpubmed-author:RobersonM SMSlld:pubmed
pubmed-article:1377511pubmed:authorpubmed-author:StumpfT TTTlld:pubmed
pubmed-article:1377511pubmed:authorpubmed-author:WerthL ALAlld:pubmed
pubmed-article:1377511pubmed:authorpubmed-author:CuppA SASlld:pubmed
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pubmed-article:1377511pubmed:volume46lld:pubmed
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pubmed-article:1377511pubmed:pagination435-41lld:pubmed
pubmed-article:1377511pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:1377511pubmed:year1992lld:pubmed
pubmed-article:1377511pubmed:articleTitleSteady-state amounts of alpha- and luteinizing hormone (LH) beta-subunit messenger ribonucleic acids are uncoupled from pulsatility of LH secretion during sexual maturation of the heifer.lld:pubmed
pubmed-article:1377511pubmed:affiliationDepartment of Animal Science, University of Nebraska-Lincoln 68583-0908.lld:pubmed
pubmed-article:1377511pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1377511pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed