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pubmed-article:1369262pubmed:dateCreated1990-10-11lld:pubmed
pubmed-article:1369262pubmed:abstractTextThe production of D-ribulose-5-phosphate in an enzyme membrane reactor was examined. Phosphoryl transfer from ATP to D-ribulose was catalysed by D-ribulokinase isolated from Klebsiella pneumoniae. For production of D-ribulose-5-phosphate the phosphoryl donor ATP was used either in stoichiometric or in catalytic amounts. Using catalytic amounts of ATP requires a second enzyme, e.g. pyruvate kinase, to regenerate ATP. The kinetic parameters for D-ribulokinase and pyruvate kinase were determined to calculate the performance of an enzyme membrane reactor for continuous production of D-ribulose-5-phosphate. Both processes operated for more than 200 h. Regardless of whether ATP was used in catalytic or stoichiometric amounts, about the same production parameters were determined. In continuous production space/time yields of 117 g (with ATP regeneration) and 103 g (without ATP regeneration) of D-ribulose-5-phosphate l -1 per day were reached.lld:pubmed
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pubmed-article:1369262pubmed:authorpubmed-author:SchärH PHPlld:pubmed
pubmed-article:1369262pubmed:authorpubmed-author:GhisalbaOOlld:pubmed
pubmed-article:1369262pubmed:authorpubmed-author:WandreyCClld:pubmed
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pubmed-article:1369262pubmed:volume32lld:pubmed
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pubmed-article:1369262pubmed:pagination621-6lld:pubmed
pubmed-article:1369262pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:1369262pubmed:year1990lld:pubmed
pubmed-article:1369262pubmed:articleTitleD-ribulokinase from Klebsiella pneumoniae for continuous production of D-(-)-ribulose-5-phosphate.lld:pubmed
pubmed-article:1369262pubmed:affiliationCentral Research Laboratories of Ciba-Geigy Ltd., Basel, Switzerland.lld:pubmed
pubmed-article:1369262pubmed:publicationTypeJournal Articlelld:pubmed