| pubmed-article:1366512 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1366512 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:1366512 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:1366512 | lifeskim:mentions | umls-concept:C0376432 | lld:lifeskim |
| pubmed-article:1366512 | lifeskim:mentions | umls-concept:C1420423 | lld:lifeskim |
| pubmed-article:1366512 | lifeskim:mentions | umls-concept:C1524063 | lld:lifeskim |
| pubmed-article:1366512 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:1366512 | pubmed:dateCreated | 1990-9-6 | lld:pubmed |
| pubmed-article:1366512 | pubmed:abstractText | Reactor takeover by plasmidless cells is a major problem encountered when producing proteins from plasmid-borne genes in genetically engineered bacteria. We have approached this problem by deleting the essential ssb gene from the Escherichia coli chromosome and placing it on a plasmid. Plasmidless cells do not accumulate even after growing such strains under non-selective continuous culture conditions for extended periods of time. Other ssb-containing plasmids can be readily introduced into this E. coli strain by a plasmid-displacement technique. Using this system, we have achieved very high levels of beta-lactamase production in continuous culture without selective pressure. | lld:pubmed |
| pubmed-article:1366512 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1366512 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1366512 | pubmed:citationSubset | B | lld:pubmed |
| pubmed-article:1366512 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1366512 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1366512 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1366512 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:1366512 | pubmed:issn | 0733-222X | lld:pubmed |
| pubmed-article:1366512 | pubmed:author | pubmed-author:PorterR DRD | lld:pubmed |
| pubmed-article:1366512 | pubmed:author | pubmed-author:BlackSS | lld:pubmed |
| pubmed-article:1366512 | pubmed:author | pubmed-author:CarlsonAA | lld:pubmed |
| pubmed-article:1366512 | pubmed:author | pubmed-author:PannuriSS | lld:pubmed |
| pubmed-article:1366512 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1366512 | pubmed:volume | 8 | lld:pubmed |
| pubmed-article:1366512 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1366512 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1366512 | pubmed:pagination | 47-51 | lld:pubmed |
| pubmed-article:1366512 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:1366512 | pubmed:meshHeading | pubmed-meshheading:1366512-... | lld:pubmed |
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| pubmed-article:1366512 | pubmed:meshHeading | pubmed-meshheading:1366512-... | lld:pubmed |
| pubmed-article:1366512 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:1366512 | pubmed:articleTitle | Use of the Escherichia coli SSB gene to prevent bioreactor takeover by plasmidless cells. | lld:pubmed |
| pubmed-article:1366512 | pubmed:affiliation | Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802. | lld:pubmed |
| pubmed-article:1366512 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1366512 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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