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pubmed-article:1359612pubmed:abstractTextDynorphin B-like immunoreactivity (ir-dyn B) was measured by a validated radio-immunoassay in gastroduodenal biopsy specimens from control and gallstone patients. Levels were significantly lower in acetic acid extracts of specimens of the transverse portion of the duodenum from gallstone patients. Gel permeation chromatography showed that almost all ir-dyn B in duodenal samples corresponded to a molecular form co-eluting with authentic dyn B. Duodenal extracts from gallstone patients had less of this form. Reverse-phase high performance liquid chromatography of the pooled gel chromatography fractions showed up a molecular form with the same retention time as synthetic dyn B which was significantly less in fractions from duodenal extracts of gallstone patients. These results indicate the occurrence of dyn B in the human gastrointestinal tract; however, at this stage of our understanding, no causal relationship can be demonstrated with functional alterations of the biliary tree.lld:pubmed
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pubmed-article:1359612pubmed:articleTitleDynorphin B-like immunoreactivity in gastroduodenal biopsy specimens from gallstone patients.lld:pubmed
pubmed-article:1359612pubmed:affiliationDepartment of Pharmacology, University of Bologna, Italy.lld:pubmed
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