| pubmed-article:1337083 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1337083 | lifeskim:mentions | umls-concept:C0085080 | lld:lifeskim |
| pubmed-article:1337083 | lifeskim:mentions | umls-concept:C0006556 | lld:lifeskim |
| pubmed-article:1337083 | lifeskim:mentions | umls-concept:C1419779 | lld:lifeskim |
| pubmed-article:1337083 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:1337083 | lifeskim:mentions | umls-concept:C0030685 | lld:lifeskim |
| pubmed-article:1337083 | lifeskim:mentions | umls-concept:C1153410 | lld:lifeskim |
| pubmed-article:1337083 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:1337083 | lifeskim:mentions | umls-concept:C0680255 | lld:lifeskim |
| pubmed-article:1337083 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:1337083 | lifeskim:mentions | umls-concept:C0391871 | lld:lifeskim |
| pubmed-article:1337083 | lifeskim:mentions | umls-concept:C1283071 | lld:lifeskim |
| pubmed-article:1337083 | lifeskim:mentions | umls-concept:C1963578 | lld:lifeskim |
| pubmed-article:1337083 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:1337083 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:1337083 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:1337083 | pubmed:dateCreated | 1993-3-4 | lld:pubmed |
| pubmed-article:1337083 | pubmed:abstractText | We constructed an expression plasmid (pMAMCRR51) that carried the entire protein-coding sequence of the rabbit cardiac ryanodine receptor cDNA, linked to the dexamethasone-inducible mouse mammary tumor virus promoter and Escherichia coli xanthine-guanine phosphoribosyltransferase (gpt). Chinese hamster ovary (CHO) cells were transfected with pMAMCRR51 and mycophenolic acid-resistant cells showing caffeine-induced intracellular Ca2+ transients were selected. Immunoprecipitation with a monoclonal antibody against the canine cardiac ryanodine receptor revealed that the cell clones thus selected exhibited Ca(2+)-dependent [3H]ryanodine binding activity, which was stimulated by 5 mM ATP or 1 M KCl. The apparent dissociation constant (Kd) for [3H]ryanodine was 6.6 nM in 1 M KCl, which was similar to the Kd obtained with cardiac microsomes. Immunoprecipitation also demonstrated that these cell clones expressed a protein indistinguishable in M(r) from the ryanodine receptor in canine cardiac microsomes. The ryanodine binding activity expressed in CHO cells increased significantly after dexamethasone induction. In saponin-skinned CHO cells transfected with pMAMCRR51, micromolar Ca2+ or millimolar caffeine evoked rapid Ca2+ release from the intracellular Ca2+ stores. In skinned control CHO cells, we did not observe such Ca2+ release activity. These results clearly demonstrate that the cardiac ryanodine receptor is stably expressed in internal membranes of CHO cells and functions as Ca(2+)-induced Ca2+ release channels. | lld:pubmed |
| pubmed-article:1337083 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1337083 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1337083 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1337083 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1337083 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:1337083 | pubmed:issn | 0021-924X | lld:pubmed |
| pubmed-article:1337083 | pubmed:author | pubmed-author:ShigekawaMM | lld:pubmed |
| pubmed-article:1337083 | pubmed:author | pubmed-author:TakeshimaHH | lld:pubmed |
| pubmed-article:1337083 | pubmed:author | pubmed-author:ImagawaTT | lld:pubmed |
| pubmed-article:1337083 | pubmed:author | pubmed-author:NakasakiYY | lld:pubmed |
| pubmed-article:1337083 | pubmed:author | pubmed-author:NakaiJJ | lld:pubmed |
| pubmed-article:1337083 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1337083 | pubmed:volume | 112 | lld:pubmed |
| pubmed-article:1337083 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1337083 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1337083 | pubmed:pagination | 508-13 | lld:pubmed |
| pubmed-article:1337083 | pubmed:dateRevised | 2007-12-19 | lld:pubmed |
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| pubmed-article:1337083 | pubmed:meshHeading | pubmed-meshheading:1337083-... | lld:pubmed |
| pubmed-article:1337083 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1337083 | pubmed:articleTitle | Expression of Ca(2+)-induced Ca2+ release channel activity from cardiac ryanodine receptor cDNA in Chinese hamster ovary cells. | lld:pubmed |
| pubmed-article:1337083 | pubmed:affiliation | Department of Molecular Physiology, National Cardiovascular Center Research Institute, Osaka. | lld:pubmed |
| pubmed-article:1337083 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1337083 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:100009536 | entrezgene:pubmed | pubmed-article:1337083 | lld:entrezgene |
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