| pubmed-article:1335457 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1335457 | lifeskim:mentions | umls-concept:C0042776 | lld:lifeskim |
| pubmed-article:1335457 | lifeskim:mentions | umls-concept:C0024794 | lld:lifeskim |
| pubmed-article:1335457 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:1335457 | lifeskim:mentions | umls-concept:C0042210 | lld:lifeskim |
| pubmed-article:1335457 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:1335457 | lifeskim:mentions | umls-concept:C0449943 | lld:lifeskim |
| pubmed-article:1335457 | lifeskim:mentions | umls-concept:C0450254 | lld:lifeskim |
| pubmed-article:1335457 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:1335457 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:1335457 | pubmed:dateCreated | 1993-1-29 | lld:pubmed |
| pubmed-article:1335457 | pubmed:abstractText | A polymerase chain reaction (PCR) test based on primers flanking the 132 bp tandem repeat in pathogenic MDV-1 DNA was developed. These primers amplify a dimer or a trimer 132 bp repeat in pathogenic MDV-1 DNA from blood and organs of commercial chickens with Marek's disease (MD) symptoms. Using the same primers in a radioactive PCR test, it was possible to distinguish between vvMDV-1 and the non-pathogenic MDV-1 CVI-988 vaccine in which the 132 bp repeats in the DNA were increased up to 9 repeats. The MDV-1 specific primers did not amplify MDV-2 (SB1) and MDV-3 (HVT) DNA. Primers prepared according to the nucleotide sequence of MDV-1 antigen A gene amplified MDV-1 DNA only. Specific primers prepared according to the nucleotide sequence of MDV-3 (HVT) antigen A gene amplified MDV-3 DNA but not MDV-1 nor MDV-2 DNA. The results of the present study show that the PCR tests can be used for the early identification of vvMDV-1 DNA in pathological samples from diseased commercial chickens and to distinguish between the vvMDV-1 and the three types of virus vaccines used to immunize chickens. The tests are accurate and can be performed in the presence of vaccine virus DNA in the sample. | lld:pubmed |
| pubmed-article:1335457 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1335457 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1335457 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1335457 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1335457 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1335457 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1335457 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:1335457 | pubmed:issn | 0166-0934 | lld:pubmed |
| pubmed-article:1335457 | pubmed:author | pubmed-author:WeismanYY | lld:pubmed |
| pubmed-article:1335457 | pubmed:author | pubmed-author:TaborEE | lld:pubmed |
| pubmed-article:1335457 | pubmed:author | pubmed-author:BeckerYY | lld:pubmed |
| pubmed-article:1335457 | pubmed:author | pubmed-author:DavidsonII | lld:pubmed |
| pubmed-article:1335457 | pubmed:author | pubmed-author:AsherYY | lld:pubmed |
| pubmed-article:1335457 | pubmed:author | pubmed-author:MalkinsonMM | lld:pubmed |
| pubmed-article:1335457 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1335457 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:1335457 | pubmed:volume | 40 | lld:pubmed |
| pubmed-article:1335457 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1335457 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1335457 | pubmed:pagination | 307-22 | lld:pubmed |
| pubmed-article:1335457 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:1335457 | pubmed:meshHeading | pubmed-meshheading:1335457-... | lld:pubmed |
| pubmed-article:1335457 | pubmed:meshHeading | pubmed-meshheading:1335457-... | lld:pubmed |
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| pubmed-article:1335457 | pubmed:meshHeading | pubmed-meshheading:1335457-... | lld:pubmed |
| pubmed-article:1335457 | pubmed:meshHeading | pubmed-meshheading:1335457-... | lld:pubmed |
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| pubmed-article:1335457 | pubmed:meshHeading | pubmed-meshheading:1335457-... | lld:pubmed |
| pubmed-article:1335457 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1335457 | pubmed:articleTitle | Polymerase chain reaction for differentiation between pathogenic and non-pathogenic serotype 1 Marek's disease viruses (MDV) and vaccine viruses of MDV-serotypes 2 and 3. | lld:pubmed |
| pubmed-article:1335457 | pubmed:affiliation | Department of Molecular Virology, Faculty of Medicine, Hebrew University of Jerusalem, Israel. | lld:pubmed |
| pubmed-article:1335457 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1335457 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:1335457 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:1335457 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1335457 | lld:pubmed |
