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pubmed-article:1332745pubmed:abstractTextTumor-infiltrating lymphocytes (TILs) were isolated from a subcutaneous metastasis of melanoma and cytotoxic T-cell lymphocyte (CTL) lines were obtained by sensitizing in vitro four separate aliquots of TILs with autologous tumor cells and recombinant interleukin-2. All CTL lines were predominantly WT31+, CD3+, and CD8+ and displayed a preferential cytotoxic activity against the autologous tumor. T-cell receptor (TCR) composition was analyzed by using the polymerase chain reaction with 5' variable region (V alpha or V beta)-specific primers and 3' constant (C alpha or C beta) primers. The entire repertoire of the V alpha and V beta gene families tested was present in fresh TILs and in the CTL lines, although, in the latter, consistent quantitative variations in transcripts of several V alpha and V beta occurred. CTL clones that exhibited CD3-dependent and major histocompatibility complex-restricted killing of the autologous melanoma were isolated from the four TIL cultures. TCR analysis indicated that, independently from the culture of origin, only two combinations of V alpha and V beta gene families were present in the majority of these CTL clones. These V alpha and V beta gene families were not found in a panel of CTL clones that did not lyse the autologous tumor. This study indicates that recognition of melanoma antigens can strongly select for certain types of TCR-bearing T-lymphocytes.lld:pubmed
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pubmed-article:1332745pubmed:pagination207-11lld:pubmed
pubmed-article:1332745pubmed:dateRevised2008-3-18lld:pubmed
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pubmed-article:1332745pubmed:year1992lld:pubmed
pubmed-article:1332745pubmed:articleTitleExpansion of major histocompatibility complex-restricted antimelanoma cytotoxic T-cell lymphocyte clones with identical T-cell receptor from tumor-infiltrating lymphocytes.lld:pubmed
pubmed-article:1332745pubmed:affiliationDivision of Experimental Oncology D, Istituto Nazionale Tumori, Milan, Italy.lld:pubmed
pubmed-article:1332745pubmed:publicationTypeJournal Articlelld:pubmed
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