| pubmed-article:1321135 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1321135 | lifeskim:mentions | umls-concept:C0038975 | lld:lifeskim |
| pubmed-article:1321135 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:1321135 | lifeskim:mentions | umls-concept:C0242961 | lld:lifeskim |
| pubmed-article:1321135 | lifeskim:mentions | umls-concept:C0003343 | lld:lifeskim |
| pubmed-article:1321135 | lifeskim:mentions | umls-concept:C0205173 | lld:lifeskim |
| pubmed-article:1321135 | lifeskim:mentions | umls-concept:C1706853 | lld:lifeskim |
| pubmed-article:1321135 | pubmed:issue | 20 | lld:pubmed |
| pubmed-article:1321135 | pubmed:dateCreated | 1992-8-14 | lld:pubmed |
| pubmed-article:1321135 | pubmed:abstractText | An initial step in the replication of simian virus (SV40) DNA is the ATP-dependent formation of a double hexamer of the SV40 large tumor (T) antigen at the SV40 DNA replication origin. In the absence of DNA, T antigen assembled into hexamers in the presence of magnesium and ATP. Hexameric T antigen was stable and could be isolated by glycerol gradient centrifugation. The ATPase activities of hexameric and monomeric T antigen isolated from parallel glycerol gradients were identical. However, while monomeric T antigen was active in the ATP-dependent binding, untwisting, unwinding, and replication of SV40 origin-containing DNA, hexameric T antigen was inactive in these reactions. Isolated hexamers incubated at 37 degrees C in the presence of ATP remained intact, but dissociated into monomers when incubated at 37 degrees C in the absence of ATP. This dissociation restored the activity of these preparations in the DNA replication reaction, indicating that hexameric T antigen is not permanently inactivated but merely assembled into a nonproductive structure. We propose that the two hexamers of T antigen at the SV40 origin assemble around the DNA from monomer T antigen in solution. This complex untwists the DNA at the origin, melting specific DNA sequences. The resulting single-stranded regions may be utilized by the T antigen helicase activity to initiate DNA unwinding bidirectionally from the origin. | lld:pubmed |
| pubmed-article:1321135 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1321135 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1321135 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1321135 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1321135 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1321135 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1321135 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:1321135 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:1321135 | pubmed:author | pubmed-author:HurwitzJJ | lld:pubmed |
| pubmed-article:1321135 | pubmed:author | pubmed-author:DarkJ FJF | lld:pubmed |
| pubmed-article:1321135 | pubmed:author | pubmed-author:EkiTT | lld:pubmed |
| pubmed-article:1321135 | pubmed:author | pubmed-author:BorowiecJ AJA | lld:pubmed |
| pubmed-article:1321135 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1321135 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:1321135 | pubmed:volume | 267 | lld:pubmed |
| pubmed-article:1321135 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1321135 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1321135 | pubmed:pagination | 14129-37 | lld:pubmed |
| pubmed-article:1321135 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:1321135 | pubmed:meshHeading | pubmed-meshheading:1321135-... | lld:pubmed |
| pubmed-article:1321135 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1321135 | pubmed:articleTitle | The simian virus 40 T antigen double hexamer assembles around the DNA at the replication origin. | lld:pubmed |
| pubmed-article:1321135 | pubmed:affiliation | Program in Molecular Biology, Sloan-Kettering Cancer Center, New York, New York 10021. | lld:pubmed |
| pubmed-article:1321135 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1321135 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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