| pubmed-article:1320194 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1320194 | lifeskim:mentions | umls-concept:C0043393 | lld:lifeskim |
| pubmed-article:1320194 | lifeskim:mentions | umls-concept:C1330957 | lld:lifeskim |
| pubmed-article:1320194 | lifeskim:mentions | umls-concept:C0079941 | lld:lifeskim |
| pubmed-article:1320194 | lifeskim:mentions | umls-concept:C1418508 | lld:lifeskim |
| pubmed-article:1320194 | lifeskim:mentions | umls-concept:C0596311 | lld:lifeskim |
| pubmed-article:1320194 | lifeskim:mentions | umls-concept:C1257759 | lld:lifeskim |
| pubmed-article:1320194 | lifeskim:mentions | umls-concept:C1261552 | lld:lifeskim |
| pubmed-article:1320194 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:1320194 | pubmed:dateCreated | 1992-8-6 | lld:pubmed |
| pubmed-article:1320194 | pubmed:abstractText | Insertion of an 18-nucleotide-long poly(G) tract into the 3'-terminal untranslated region of yeast phosphoglycerate kinase (PGK1) mRNA increases its chemical half-life by about a factor of 2 (P. Vreken, R. Van der Veen, V. C. H. F. de Regt, A. L. de Maat, R. J. Planta, and H. A. Raué, Biochimie 73:729-737, 1991). In this report, we show that this insertion also causes the accumulation of a degradation intermediate extending from the poly(G) sequence down to the transcription termination site. Reverse transcription and S1 nuclease mapping experiments demonstrated that this intermediate is the product of shorter-lived primary fragments resulting from endonucleolytic cleavage immediately downstream from the U residue of either of two 5'-GGUG-3' sequences present between positions 1100 and 1200 close to the 3' terminus (position 1251) of the coding sequence. Similar endonucleolytic cleavages appear to initiate degradation of wild-type PGK1 mRNA. Insertion of a poly(G) tract just upstream from the AUG start codon resulted in the accumulation of a 5'-terminal degradation intermediate extending from the insertion to the 1100-1200 region. RNase H degradation in the presence of oligo(dT) demonstrated that the wild-type and mutant PGK1 mRNAs are deadenylated prior to endonucleolytic cleavage and that the half-life of the poly(A) tail is three- to sixfold lower than that of the remainder of the mRNA. Thus, the endonucleolytic cleavage constitutes the rate-limiting step in degradation of both wild-type and mutant PGK1 transcripts, and the resulting fragments are degraded by a 5'----3' exonuclease, which appears to be severely retarded by a poly(G) sequence. | lld:pubmed |
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| pubmed-article:1320194 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1320194 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1320194 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1320194 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1320194 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1320194 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:1320194 | pubmed:issn | 0270-7306 | lld:pubmed |
| pubmed-article:1320194 | pubmed:author | pubmed-author:RauéH AHA | lld:pubmed |
| pubmed-article:1320194 | pubmed:author | pubmed-author:VrekenPP | lld:pubmed |
| pubmed-article:1320194 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1320194 | pubmed:volume | 12 | lld:pubmed |
| pubmed-article:1320194 | pubmed:geneSymbol | PCK1 | lld:pubmed |
| pubmed-article:1320194 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1320194 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1320194 | pubmed:pagination | 2986-96 | lld:pubmed |
| pubmed-article:1320194 | pubmed:dateRevised | 2010-9-7 | lld:pubmed |
| pubmed-article:1320194 | pubmed:meshHeading | pubmed-meshheading:1320194-... | lld:pubmed |
| pubmed-article:1320194 | pubmed:meshHeading | pubmed-meshheading:1320194-... | lld:pubmed |
