| pubmed-article:1320046 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1320046 | lifeskim:mentions | umls-concept:C0085080 | lld:lifeskim |
| pubmed-article:1320046 | lifeskim:mentions | umls-concept:C1325834 | lld:lifeskim |
| pubmed-article:1320046 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:1320046 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:1320046 | pubmed:dateCreated | 1992-8-5 | lld:pubmed |
| pubmed-article:1320046 | pubmed:abstractText | Truncated Epstein-Barr virus (EBV) membrane antigen gp350/220 (EBV-MA) lacking the membrane anchor was expressed and secreted into the medium of recombinant Chinese hamster ovary cells that had been cultured in Plasmapur hollow-fibre modules using defined serum-free medium. The EBV-MA in the medium was concentrated by 70% (w/v) ammonium sulphate precipitation and subsequently purified by immunoaffinity chromatography using an anti-EBV-MA (EBV.0T6) monoclonal antibody (mAb) column. Adsorbed antigen was eluted with 3 M MgCl2 in phosphate-buffered saline, concentrated by Mono Q anion-exchange chromatography and analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, silver staining and Western blotting using EBV-positive serum and anti-EBV-MA specific mAbs. Monospecific polyclonal rabbit antibodies against the purified EBV-MA were raised and purified by protein G affinity chromatography. For the measurement of EBV-MA antigen levels a sandwich enzyme-linked immunosorbent assay using rabbit polyclonal antibodies and a horseradish peroxidase-conjugated anti-MA mAb was developed having a detection level of 10 ng/ml. | lld:pubmed |
| pubmed-article:1320046 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1320046 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1320046 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1320046 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1320046 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1320046 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1320046 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1320046 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1320046 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1320046 | pubmed:month | May | lld:pubmed |
| pubmed-article:1320046 | pubmed:issn | 0021-9673 | lld:pubmed |
| pubmed-article:1320046 | pubmed:author | pubmed-author:WolfHH | lld:pubmed |
| pubmed-article:1320046 | pubmed:author | pubmed-author:MiddeldorpJ... | lld:pubmed |
| pubmed-article:1320046 | pubmed:author | pubmed-author:HessingMM | lld:pubmed |
| pubmed-article:1320046 | pubmed:author | pubmed-author:van... | lld:pubmed |
| pubmed-article:1320046 | pubmed:author | pubmed-author:van... | lld:pubmed |
| pubmed-article:1320046 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1320046 | pubmed:day | 22 | lld:pubmed |
| pubmed-article:1320046 | pubmed:volume | 599 | lld:pubmed |
| pubmed-article:1320046 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1320046 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1320046 | pubmed:pagination | 267-72 | lld:pubmed |
| pubmed-article:1320046 | pubmed:dateRevised | 2005-11-17 | lld:pubmed |
| pubmed-article:1320046 | pubmed:meshHeading | pubmed-meshheading:1320046-... | lld:pubmed |
| pubmed-article:1320046 | pubmed:meshHeading | pubmed-meshheading:1320046-... | lld:pubmed |
| pubmed-article:1320046 | pubmed:meshHeading | pubmed-meshheading:1320046-... | lld:pubmed |
| pubmed-article:1320046 | pubmed:meshHeading | pubmed-meshheading:1320046-... | lld:pubmed |
| pubmed-article:1320046 | pubmed:meshHeading | pubmed-meshheading:1320046-... | lld:pubmed |
| pubmed-article:1320046 | pubmed:meshHeading | pubmed-meshheading:1320046-... | lld:pubmed |
| pubmed-article:1320046 | pubmed:meshHeading | pubmed-meshheading:1320046-... | lld:pubmed |
| pubmed-article:1320046 | pubmed:meshHeading | pubmed-meshheading:1320046-... | lld:pubmed |
| pubmed-article:1320046 | pubmed:meshHeading | pubmed-meshheading:1320046-... | lld:pubmed |
| pubmed-article:1320046 | pubmed:meshHeading | pubmed-meshheading:1320046-... | lld:pubmed |
| pubmed-article:1320046 | pubmed:meshHeading | pubmed-meshheading:1320046-... | lld:pubmed |
| pubmed-article:1320046 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1320046 | pubmed:articleTitle | Purification and quantification of recombinant Epstein-Barr viral glycoproteins gp350/220 from Chinese hamster ovary cells. | lld:pubmed |
| pubmed-article:1320046 | pubmed:affiliation | Biotechnological Research Unit, Organon Teknika, Boxtel, Netherlands. | lld:pubmed |
| pubmed-article:1320046 | pubmed:publicationType | Journal Article | lld:pubmed |
