| pubmed-article:1309780 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1309780 | lifeskim:mentions | umls-concept:C0229671 | lld:lifeskim |
| pubmed-article:1309780 | lifeskim:mentions | umls-concept:C0282560 | lld:lifeskim |
| pubmed-article:1309780 | lifeskim:mentions | umls-concept:C1622186 | lld:lifeskim |
| pubmed-article:1309780 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:1309780 | lifeskim:mentions | umls-concept:C0678640 | lld:lifeskim |
| pubmed-article:1309780 | lifeskim:mentions | umls-concept:C0597484 | lld:lifeskim |
| pubmed-article:1309780 | lifeskim:mentions | umls-concept:C0851827 | lld:lifeskim |
| pubmed-article:1309780 | lifeskim:mentions | umls-concept:C1701901 | lld:lifeskim |
| pubmed-article:1309780 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:1309780 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:1309780 | pubmed:dateCreated | 1992-2-14 | lld:pubmed |
| pubmed-article:1309780 | pubmed:abstractText | Regulation of Na+/H+ exchange by fetal bovine serum was studied in Caco-2 cells, an established cell line derived from a human colon carcinoma. Cells were grown as polarized monolayers on collagen-coated filters and intracellular pH measured fluorometrically with 2',7'-bis(2-carboxymethyl)-5,6-carboxyfluorescein. Na+/H+ exchange was reduced 64% when cells were deprived of serum for 4 h. In contrast to other cell types, readdition of serum for 10 min did not activate Na+/H+ exchange; however, readdition of serum for 4 h restored Na+/H+ exchange to control values. This long-term effect of serum on Na+/H+ exchange activity could not be explained by changes in intracellular buffering capacity or intracellular [Na+]. 4-h serum deprivation reduced the K(t) of the exchanger for external Na+ from 21 to 6 mM, and reduced the V(max) by 57%, but did not alter the IC50 for amiloride in the presence of 140 mM Na+. Inhibition of protein synthesis with cycloheximide (5 microM) did not alter the effect of serum removal or readdition on Na+/H+ exchange. Low temperature (13 degrees C) completely prevented the inhibition of Na+/H+ exchange caused by the removal of serum. In addition, once Na+/H+ exchange was inhibited by serum removal at 37 degrees C, maintaining cells at 13 degrees C also blocked the recovery of Na+/H+ exchange caused by serum readdition. Conversely, cytochalasin D (0.1-20 microM) blocked the reduction of Na+/H+ exchange which occurred due to 4-h serum deprivation, but did not block the restoration of Na+/H+ exchange when the cells were re-exposed to serum for a further 4 h. Colchicine (20 microM) did not alter the effect of serum removal or readdition. These data suggest that serum regulates Na+/H+ exchange activity by a posttranslational mechanism which is dependent on F-actin. | lld:pubmed |
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| pubmed-article:1309780 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1309780 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1309780 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1309780 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1309780 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1309780 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:1309780 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:1309780 | pubmed:author | pubmed-author:LevineSS | lld:pubmed |
| pubmed-article:1309780 | pubmed:author | pubmed-author:WatsonA JAJ | lld:pubmed |
| pubmed-article:1309780 | pubmed:author | pubmed-author:DonowitzMM | lld:pubmed |
| pubmed-article:1309780 | pubmed:author | pubmed-author:MontroseM HMH | lld:pubmed |
| pubmed-article:1309780 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1309780 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:1309780 | pubmed:volume | 267 | lld:pubmed |
| pubmed-article:1309780 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1309780 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1309780 | pubmed:pagination | 956-62 | lld:pubmed |
| pubmed-article:1309780 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:1309780 | pubmed:meshHeading | pubmed-meshheading:1309780-... | lld:pubmed |
| pubmed-article:1309780 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1309780 | pubmed:articleTitle | Serum regulates Na+/H+ exchange in Caco-2 cells by a mechanism which is dependent on F-actin. | lld:pubmed |
| pubmed-article:1309780 | pubmed:affiliation | Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205. | lld:pubmed |
| pubmed-article:1309780 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1309780 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1309780 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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