| pubmed-article:12972635 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12972635 | lifeskim:mentions | umls-concept:C0030657 | lld:lifeskim |
| pubmed-article:12972635 | lifeskim:mentions | umls-concept:C0019682 | lld:lifeskim |
| pubmed-article:12972635 | lifeskim:mentions | umls-concept:C0019699 | lld:lifeskim |
| pubmed-article:12972635 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:12972635 | lifeskim:mentions | umls-concept:C0317589 | lld:lifeskim |
| pubmed-article:12972635 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
| pubmed-article:12972635 | lifeskim:mentions | umls-concept:C0021467 | lld:lifeskim |
| pubmed-article:12972635 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:12972635 | lifeskim:mentions | umls-concept:C1332714 | lld:lifeskim |
| pubmed-article:12972635 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:12972635 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
| pubmed-article:12972635 | lifeskim:mentions | umls-concept:C0370215 | lld:lifeskim |
| pubmed-article:12972635 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
| pubmed-article:12972635 | lifeskim:mentions | umls-concept:C0205296 | lld:lifeskim |
| pubmed-article:12972635 | pubmed:issue | 20 | lld:pubmed |
| pubmed-article:12972635 | pubmed:dateCreated | 2003-10-1 | lld:pubmed |
| pubmed-article:12972635 | pubmed:abstractText | The predominant mode of HIV transmission worldwide is via heterosexual contact, with the cervico-vaginal mucosa being the main portal of entry in women. The cervico-vaginal mucosa is naturally colonized with commensal bacteria, primarily lactobacilli. To address the urgent need for female-controlled approaches to block the heterosexual transmission of HIV, we have engineered natural human vaginal isolates of Lactobacillus jensenii to secrete two-domain CD4 (2D CD4) proteins. The secreted 2D CD4 recognized a conformation-dependent anti-CD4 antibody and bound HIV type 1 (HIV-1) gp120, suggesting that the expressed proteins adopted a native conformation. Single-cycle infection assays using HIV-1HxB2 carrying a luciferase reporter gene demonstrated that Lactobacillus-derived 2D CD4 inhibited HIV-1 entry into target cells in a dose-dependent manner. Importantly, coincubation of the engineered bacteria with recombinant HIV-1HxB2 reporter virus led to a significant decrease in virus infectivity of HeLa cells expressing CD4-CXCR4-CCR5. Engineered lactobacilli also caused a modest, but statistically significant, decrease in infectivity of a primary isolate, HIV-1JR-FL. This represents an important first step toward the development of engineered commensal bacteria within the vaginal microflora to inhibit heterosexual transmission of HIV. | lld:pubmed |
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| pubmed-article:12972635 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12972635 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12972635 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12972635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:12972635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12972635 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12972635 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:12972635 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:12972635 | pubmed:author | pubmed-author:XuQiangQ | lld:pubmed |
| pubmed-article:12972635 | pubmed:author | pubmed-author:SchoolnikGary... | lld:pubmed |
| pubmed-article:12972635 | pubmed:author | pubmed-author:HoDavid DDD | lld:pubmed |
| pubmed-article:12972635 | pubmed:author | pubmed-author:LeePeter PPP | lld:pubmed |
| pubmed-article:12972635 | pubmed:author | pubmed-author:HillierSharon... | lld:pubmed |
| pubmed-article:12972635 | pubmed:author | pubmed-author:HolodniyMarkM | lld:pubmed |
| pubmed-article:12972635 | pubmed:author | pubmed-author:LewickiJohn... | lld:pubmed |
| pubmed-article:12972635 | pubmed:author | pubmed-author:ChangTheresa... | lld:pubmed |
| pubmed-article:12972635 | pubmed:author | pubmed-author:ChangChia-Hwa... | lld:pubmed |
| pubmed-article:12972635 | pubmed:author | pubmed-author:SimpsonDavid... | lld:pubmed |
| pubmed-article:12972635 | pubmed:author | pubmed-author:MartinPatrick... | lld:pubmed |
| pubmed-article:12972635 | pubmed:author | pubmed-author:LagenaurLaure... | lld:pubmed |
| pubmed-article:12972635 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12972635 | pubmed:day | 30 | lld:pubmed |
| pubmed-article:12972635 | pubmed:volume | 100 | lld:pubmed |
| pubmed-article:12972635 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12972635 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12972635 | pubmed:pagination | 11672-7 | lld:pubmed |
| pubmed-article:12972635 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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