| pubmed-article:12972296 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12972296 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:12972296 | lifeskim:mentions | umls-concept:C0011777 | lld:lifeskim |
| pubmed-article:12972296 | lifeskim:mentions | umls-concept:C1159825 | lld:lifeskim |
| pubmed-article:12972296 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:12972296 | lifeskim:mentions | umls-concept:C0591833 | lld:lifeskim |
| pubmed-article:12972296 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:12972296 | pubmed:dateCreated | 2003-9-15 | lld:pubmed |
| pubmed-article:12972296 | pubmed:abstractText | Glucocorticoids are widely used as anti-inflammatory and chemotherapeutic agents. However, prolonged use of glucocorticoids leads to osteoporosis. This study was designed to examine the mechanism of dexamethasone (DEX)-induced apoptosis in murine osteoblastic MC3T3-E1 cells. Total RNA was extracted from MC3T3-E1 cells treated with 10(-7) M DEX for 6 h. DEX exerted a variety of effects on apoptotic gene expression in osteoblasts. Ribonuclease protection assays (RPA) revealed that DEX upregulated mRNA levels of caspases-1, -3, -6, -8, -11, -12, and bcl-XL. Western blot analysis showed enhanced processing of these caspases, with the appearance of their activated enzymes 8 h after DEX treatment. In addition, DEX also induced the activation of caspase-9. DEX elevated the levels of cleaved poly(ADP-ribose) polymerase and lamin A, a caspase-3 and a caspase-6 substrate, respectively. Expression of bcl-XL protein level was upregulated by DEX. Cytochrome c release was detected in the cytosol of DEX-treated cells. Furthermore, caspase-3 enzyme activity was elevated by 2-fold after DEX treatment for 7 h. Finally, early apoptotic cells were detected in cells treated with DEX for 3 h. Our results demonstrate that DEX-induced apoptosis involves gene activation of a number of caspases. | lld:pubmed |
| pubmed-article:12972296 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12972296 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12972296 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12972296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12972296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12972296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12972296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12972296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12972296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12972296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12972296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12972296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12972296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12972296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12972296 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12972296 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:12972296 | pubmed:issn | 0006-3002 | lld:pubmed |
| pubmed-article:12972296 | pubmed:author | pubmed-author:HamdyRonald... | lld:pubmed |
| pubmed-article:12972296 | pubmed:author | pubmed-author:ChenZhongyiZ | lld:pubmed |
| pubmed-article:12972296 | pubmed:author | pubmed-author:ChuaChu... | lld:pubmed |
| pubmed-article:12972296 | pubmed:author | pubmed-author:ChuaBalvin... | lld:pubmed |
| pubmed-article:12972296 | pubmed:author | pubmed-author:LandyCathyC | lld:pubmed |
| pubmed-article:12972296 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12972296 | pubmed:day | 23 | lld:pubmed |
| pubmed-article:12972296 | pubmed:volume | 1642 | lld:pubmed |
| pubmed-article:12972296 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12972296 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12972296 | pubmed:pagination | 79-85 | lld:pubmed |
| pubmed-article:12972296 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:12972296 | pubmed:meshHeading | pubmed-meshheading:12972296... | lld:pubmed |
| pubmed-article:12972296 | pubmed:meshHeading | pubmed-meshheading:12972296... | lld:pubmed |
| pubmed-article:12972296 | pubmed:meshHeading | pubmed-meshheading:12972296... | lld:pubmed |
| pubmed-article:12972296 | pubmed:meshHeading | pubmed-meshheading:12972296... | lld:pubmed |
| pubmed-article:12972296 | pubmed:meshHeading | pubmed-meshheading:12972296... | lld:pubmed |
| pubmed-article:12972296 | pubmed:meshHeading | pubmed-meshheading:12972296... | lld:pubmed |
| pubmed-article:12972296 | pubmed:meshHeading | pubmed-meshheading:12972296... | lld:pubmed |
| pubmed-article:12972296 | pubmed:meshHeading | pubmed-meshheading:12972296... | lld:pubmed |
| pubmed-article:12972296 | pubmed:meshHeading | pubmed-meshheading:12972296... | lld:pubmed |
| pubmed-article:12972296 | pubmed:meshHeading | pubmed-meshheading:12972296... | lld:pubmed |
| pubmed-article:12972296 | pubmed:meshHeading | pubmed-meshheading:12972296... | lld:pubmed |
| pubmed-article:12972296 | pubmed:meshHeading | pubmed-meshheading:12972296... | lld:pubmed |
| pubmed-article:12972296 | pubmed:meshHeading | pubmed-meshheading:12972296... | lld:pubmed |
| pubmed-article:12972296 | pubmed:meshHeading | pubmed-meshheading:12972296... | lld:pubmed |
| pubmed-article:12972296 | pubmed:meshHeading | pubmed-meshheading:12972296... | lld:pubmed |
| pubmed-article:12972296 | pubmed:meshHeading | pubmed-meshheading:12972296... | lld:pubmed |
| pubmed-article:12972296 | pubmed:meshHeading | pubmed-meshheading:12972296... | lld:pubmed |
| pubmed-article:12972296 | pubmed:meshHeading | pubmed-meshheading:12972296... | lld:pubmed |
| pubmed-article:12972296 | pubmed:meshHeading | pubmed-meshheading:12972296... | lld:pubmed |
| pubmed-article:12972296 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12972296 | pubmed:articleTitle | Dexamethasone induces caspase activation in murine osteoblastic MC3T3-E1 cells. | lld:pubmed |
| pubmed-article:12972296 | pubmed:affiliation | Osteoporosis Center, James H. Quillen College of Medicine, East Tennessee State University, and Veterans Affairs Medical Center, Box 70432, Johnson City, TN 37614, USA. chua.chu@med.va.gov | lld:pubmed |
| pubmed-article:12972296 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12972296 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:12972296 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12972296 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12972296 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12972296 | lld:pubmed |
