| pubmed-article:12962625 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12962625 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:12962625 | lifeskim:mentions | umls-concept:C0010423 | lld:lifeskim |
| pubmed-article:12962625 | lifeskim:mentions | umls-concept:C1999177 | lld:lifeskim |
| pubmed-article:12962625 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:12962625 | pubmed:dateCreated | 2003-9-9 | lld:pubmed |
| pubmed-article:12962625 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12962625 | pubmed:abstractText | Suitable conditions for protein crystallization are commonly identified by screening combinations of independent factors that affect crystal formation. Because precipitating agents are prime determinants of crystallization, we investigated whether a systematic exploration of combinations of mechanistically distinct precipitants would enhance crystallization. A crystallization screen containing 64 precipitant mixtures was devised. Tests with ten HIV envelope-related proteins demonstrated that use of precipitant mixtures significantly enhanced both the probability of crystallization as well as the quality of optimized crystals. Tests with hen egg white lysozyme generated a novel C2 crystal from a salt/organic solvent mixture; structure solution at 2 A resolution revealed a lattice held together by both hydrophobic and electrostatic dyad interactions. The results indicate that mechanistically distinct precipitants can synergize, with precipitant combinations adding unique dimensions to protein crystallization. | lld:pubmed |
| pubmed-article:12962625 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12962625 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12962625 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12962625 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12962625 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12962625 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12962625 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:12962625 | pubmed:issn | 0969-2126 | lld:pubmed |
| pubmed-article:12962625 | pubmed:author | pubmed-author:HuangChih-chi... | lld:pubmed |
| pubmed-article:12962625 | pubmed:author | pubmed-author:KwongPeter... | lld:pubmed |
| pubmed-article:12962625 | pubmed:author | pubmed-author:ZhouTongqingT | lld:pubmed |
| pubmed-article:12962625 | pubmed:author | pubmed-author:MajeedShahzad... | lld:pubmed |
| pubmed-article:12962625 | pubmed:author | pubmed-author:OfekGiladG | lld:pubmed |
| pubmed-article:12962625 | pubmed:author | pubmed-author:BelachewAdamA | lld:pubmed |
| pubmed-article:12962625 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12962625 | pubmed:volume | 11 | lld:pubmed |
| pubmed-article:12962625 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12962625 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12962625 | pubmed:pagination | 1061-70 | lld:pubmed |
| pubmed-article:12962625 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:12962625 | pubmed:meshHeading | pubmed-meshheading:12962625... | lld:pubmed |
| pubmed-article:12962625 | pubmed:meshHeading | pubmed-meshheading:12962625... | lld:pubmed |
| pubmed-article:12962625 | pubmed:meshHeading | pubmed-meshheading:12962625... | lld:pubmed |
| pubmed-article:12962625 | pubmed:meshHeading | pubmed-meshheading:12962625... | lld:pubmed |
| pubmed-article:12962625 | pubmed:meshHeading | pubmed-meshheading:12962625... | lld:pubmed |
| pubmed-article:12962625 | pubmed:meshHeading | pubmed-meshheading:12962625... | lld:pubmed |
| pubmed-article:12962625 | pubmed:meshHeading | pubmed-meshheading:12962625... | lld:pubmed |
| pubmed-article:12962625 | pubmed:meshHeading | pubmed-meshheading:12962625... | lld:pubmed |
| pubmed-article:12962625 | pubmed:meshHeading | pubmed-meshheading:12962625... | lld:pubmed |
| pubmed-article:12962625 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12962625 | pubmed:articleTitle | Enhancing protein crystallization through precipitant synergy. | lld:pubmed |
| pubmed-article:12962625 | pubmed:affiliation | Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA. | lld:pubmed |
| pubmed-article:12962625 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12962625 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:12962625 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:12962625 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12962625 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12962625 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12962625 | lld:pubmed |
