| pubmed-article:12944181 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12944181 | lifeskim:mentions | umls-concept:C0025932 | lld:lifeskim |
| pubmed-article:12944181 | lifeskim:mentions | umls-concept:C0229671 | lld:lifeskim |
| pubmed-article:12944181 | lifeskim:mentions | umls-concept:C0020197 | lld:lifeskim |
| pubmed-article:12944181 | pubmed:issue | 3-4 | lld:pubmed |
| pubmed-article:12944181 | pubmed:dateCreated | 2003-8-28 | lld:pubmed |
| pubmed-article:12944181 | pubmed:abstractText | Cancer cell lines often secrete hyaluronidase, suggesting that this enzyme could be used as a marker of growing tumours. We have measured hyaluronidase in the sera of non-grafted mice and mice grafted with human tumour-derived hyaluronidase-secreting H460M and SA87 cells or non-secreting CB 193 cells. Mouse serum hyaluronidase was measured at pH 3.8 using the enzyme-linked sorbent assay (ELSA) technique by reference to human serum whose activity at pH 3.8 was determined by the Reissig technique. The serum hyaluronidase in non-grafted mice ranged from 310-520 mU l(-1) (mean+/-SD 432+/-70 mU l(-1), median 440 mU l(-1)). Hyaluronidase increased in the sera of tumour-bearing mice grafted with H460M cells or with SA87 cells, but not in the sera of mice grafted with CB 193 cells. Serum hyaluronidase activity in H460M or SA87 tumour-bearing mice correlated with the tumour mass, increased with time, and decreased after tumour removal. Zymography detected two different hyaluronidase forms in the sera of non-grafted mice: type 1 had only one hyaluronidase band and type 2 had five different bands. In both types, enzyme augmentation in tumour-bearing mice correlated with the presence of an additional enzyme band that was not seen in normal sera and that migrated as the cancer cell enzyme did; there was no augmentation of the normal isoform(s). These results show that serum hyaluronidase can be used to follow the development of tumours in mice grafted with hyaluronidase-secreting cells. | lld:pubmed |
| pubmed-article:12944181 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12944181 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12944181 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12944181 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12944181 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12944181 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12944181 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12944181 | pubmed:issn | 1354-750X | lld:pubmed |
| pubmed-article:12944181 | pubmed:author | pubmed-author:SesboüéRichar... | lld:pubmed |
| pubmed-article:12944181 | pubmed:author | pubmed-author:DelpechBertra... | lld:pubmed |
| pubmed-article:12944181 | pubmed:author | pubmed-author:CourelMarie-N... | lld:pubmed |
| pubmed-article:12944181 | pubmed:author | pubmed-author:MaingonnatCat... | lld:pubmed |
| pubmed-article:12944181 | pubmed:author | pubmed-author:VincentJean-C... | lld:pubmed |
| pubmed-article:12944181 | pubmed:author | pubmed-author:BertrandPhili... | lld:pubmed |
| pubmed-article:12944181 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12944181 | pubmed:volume | 8 | lld:pubmed |
| pubmed-article:12944181 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12944181 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12944181 | pubmed:pagination | 333-8 | lld:pubmed |
| pubmed-article:12944181 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12944181 | pubmed:meshHeading | pubmed-meshheading:12944181... | lld:pubmed |
| pubmed-article:12944181 | pubmed:meshHeading | pubmed-meshheading:12944181... | lld:pubmed |
| pubmed-article:12944181 | pubmed:meshHeading | pubmed-meshheading:12944181... | lld:pubmed |
| pubmed-article:12944181 | pubmed:meshHeading | pubmed-meshheading:12944181... | lld:pubmed |
| pubmed-article:12944181 | pubmed:meshHeading | pubmed-meshheading:12944181... | lld:pubmed |
| pubmed-article:12944181 | pubmed:meshHeading | pubmed-meshheading:12944181... | lld:pubmed |
| pubmed-article:12944181 | pubmed:meshHeading | pubmed-meshheading:12944181... | lld:pubmed |
| pubmed-article:12944181 | pubmed:meshHeading | pubmed-meshheading:12944181... | lld:pubmed |
| pubmed-article:12944181 | pubmed:meshHeading | pubmed-meshheading:12944181... | lld:pubmed |
| pubmed-article:12944181 | pubmed:meshHeading | pubmed-meshheading:12944181... | lld:pubmed |
| pubmed-article:12944181 | pubmed:meshHeading | pubmed-meshheading:12944181... | lld:pubmed |
| pubmed-article:12944181 | pubmed:articleTitle | Hyaluronidase in sera of tumour-bearing nude mice. | lld:pubmed |
| pubmed-article:12944181 | pubmed:affiliation | Department of Molecular Oncology, Centre Henri-Becquerel, Rue d'Amiens, 76000 Rouen, France. catherine.maingonnat@rouen.fnclcc.fr | lld:pubmed |
| pubmed-article:12944181 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12944181 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
