| pubmed-article:12941839 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12941839 | lifeskim:mentions | umls-concept:C1140680 | lld:lifeskim |
| pubmed-article:12941839 | lifeskim:mentions | umls-concept:C1704689 | lld:lifeskim |
| pubmed-article:12941839 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:12941839 | lifeskim:mentions | umls-concept:C1510884 | lld:lifeskim |
| pubmed-article:12941839 | lifeskim:mentions | umls-concept:C0598934 | lld:lifeskim |
| pubmed-article:12941839 | pubmed:issue | 16 | lld:pubmed |
| pubmed-article:12941839 | pubmed:dateCreated | 2003-8-27 | lld:pubmed |
| pubmed-article:12941839 | pubmed:abstractText | Interleukin-10 (IL-10) is an immunosuppressive cytokine produced by T lymphocytes and drawing attention as an inhibitor of tumor angiogenesis. In this study, we investigated antiangiogenic and tumor suppressive effects of IL-10 in ovarian cancer cells. mIL-10-expressing plasmid was transferred into two ovarian cancer cell lines, SHIN-3 [vascular endothelial growth factor (VEGF) producing] and KOC-2S (non-VEGF producing). After selection, mIL-10-expressing cells were obtained as SHIN-3/mIL-10 and KOC-2S/mIL-10. No significant differences were observed in in vitro growth properties between mIL-10-expressing cells and control (luciferase expressing) cells in either KOC-2S or SHIN-3. The angiogenic activities of mIL-10-expressing cells were measured by dorsal air sac assay, which detected the number of newly formed blood vessels within a chamber in vivo. In addition, tumor formation was evaluated by s.c. tumor transplantation, and survival was monitored after i.p. injection of ovarian cancer cells into BALB/c nude mice. Both in vivo angiogenic activity and tumor growth were significantly inhibited in SHIN-3/mIL-10 cells compared with the control. Moreover, peritoneal dissemination was inhibited, and the survival period was significantly prolonged (mean survival days > 90 versus 36). In contrast, in the case of KOC-2S cells, no significant differences were observed in any of the parameters tested. These results indicate that IL-10 has suppressive effects on angiogenesis, tumor growth, and peritoneal dissemination of VEGF-producing ovarian cancer cells. Although the mechanisms of the antiangiogenic effect of IL-10 are still unclear, the potential usefulness of IL-10-mediated gene therapy of ovarian cancer was suggested. | lld:pubmed |
| pubmed-article:12941839 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12941839 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12941839 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12941839 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12941839 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12941839 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12941839 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12941839 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12941839 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12941839 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12941839 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:12941839 | pubmed:issn | 0008-5472 | lld:pubmed |
| pubmed-article:12941839 | pubmed:author | pubmed-author:SatoIkuoI | lld:pubmed |
| pubmed-article:12941839 | pubmed:author | pubmed-author:OzawaKeiyaK | lld:pubmed |
| pubmed-article:12941839 | pubmed:author | pubmed-author:SuzukiMitsuak... | lld:pubmed |
| pubmed-article:12941839 | pubmed:author | pubmed-author:KohnoTakahiro... | lld:pubmed |
| pubmed-article:12941839 | pubmed:author | pubmed-author:SagaYasushiY | lld:pubmed |
| pubmed-article:12941839 | pubmed:author | pubmed-author:TakeiYujiY | lld:pubmed |
| pubmed-article:12941839 | pubmed:author | pubmed-author:MizukamiHiroa... | lld:pubmed |
| pubmed-article:12941839 | pubmed:author | pubmed-author:KumeAkihiroA | lld:pubmed |
| pubmed-article:12941839 | pubmed:author | pubmed-author:OkadaTakashiT | lld:pubmed |
| pubmed-article:12941839 | pubmed:author | pubmed-author:MatsushitaTak... | lld:pubmed |
| pubmed-article:12941839 | pubmed:author | pubmed-author:HanazonoYutak... | lld:pubmed |
| pubmed-article:12941839 | pubmed:author | pubmed-author:ShimpoMasahis... | lld:pubmed |
| pubmed-article:12941839 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12941839 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:12941839 | pubmed:volume | 63 | lld:pubmed |
| pubmed-article:12941839 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12941839 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12941839 | pubmed:pagination | 5091-4 | lld:pubmed |
| pubmed-article:12941839 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:12941839 | pubmed:meshHeading | pubmed-meshheading:12941839... | lld:pubmed |
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| pubmed-article:12941839 | pubmed:meshHeading | pubmed-meshheading:12941839... | lld:pubmed |
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| pubmed-article:12941839 | pubmed:meshHeading | pubmed-meshheading:12941839... | lld:pubmed |
| pubmed-article:12941839 | pubmed:meshHeading | pubmed-meshheading:12941839... | lld:pubmed |
| pubmed-article:12941839 | pubmed:meshHeading | pubmed-meshheading:12941839... | lld:pubmed |
| pubmed-article:12941839 | pubmed:meshHeading | pubmed-meshheading:12941839... | lld:pubmed |
| pubmed-article:12941839 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12941839 | pubmed:articleTitle | Interleukin-10-mediated inhibition of angiogenesis and tumor growth in mice bearing VEGF-producing ovarian cancer. | lld:pubmed |
| pubmed-article:12941839 | pubmed:affiliation | Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Tochigi, 329-0498 Japan. | lld:pubmed |
| pubmed-article:12941839 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12941839 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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